This study prepared immobilized Alishewanella sp. WH16-1 using alginate and lotus seed pods as a matrix and investigated the effects of its immobilization on Cd²⁺ in a culture solution and in soil. Compared with the free WH16-1 strain, the immobilized WH16-1 strain possessed greater stability for long-term use and storage and higher removal ability for Cd²⁺ in the culture solution. A model of Cd²⁺ removal by the immobilized WH16-1 strain was proposed. The immobilized WH16-1 strain was incubated in the pot experiments of Cd-contaminated paddy soil for 120 days, and the pot experiments of Cd-contaminated paddy soil without the immobilized WH16-1 strain were used as a control. Compared with the control, the exchangeable and carbonate-bound Cd in the paddy soil incubated with the immobilized WH16-1 strain significantly decreased by 33.6% (P < 0.05) and 17.36%, respectively, and the Cd concentrations in the rice significantly decreased by 78.31% (P < 0.05). The results indicate that alginate-lotus seed pods can be used as excellent cost-effective cell carriers for the immobilization of Alishewanella sp. WH16-1 and that the immobilized WH16-1 strain may be applicable for the biological stabilization of Cd in Cd-contaminated paddy soil.

本研究制备了固定化的Alishewanella sp.。WH16-1以藻酸盐和莲子荚为基质，研究了固定化对培养液和土壤中Cd ²⁺的影响。与游离的WH16-1菌株相比，固定化的WH16-1菌株在长期使用和储存时具有更高的稳定性，并且在培养液中对Cd ^2+的去除能力更高。Cd ²⁺的模型有人提出用固定化的WH16-1菌株去除细菌。将固定化的WH16-1菌株在Cd污染的水稻土的盆栽实验中孵育120天，并将没有固定化的WH16-1菌株的Cd污染的水稻土的盆栽实验作为对照。与对照相比，固定化WH16-1菌株培养的水稻土中可交换的Cd和与碳酸盐结合的Cd分别显着降低了33.6％（P <0.05）和17.36％，而水稻中Cd的浓度显着降低了。 78.31％（P <0.05）。结果表明，藻酸盐-莲子荚可以用作固定Alishewanella的极具成本效益的细胞载体sp。WH16-1和固定化的WH16-1菌株可能适用于Cd污染的稻田土壤中Cd的生物稳定化。