均相肾上腺素-黑色素溶液的形成，以制造用于增强光电化学生物传感的电极,Langmuir

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均相肾上腺素-黑色素溶液的形成，以制造用于增强光电化学生物传感的电极
Langmuir ( IF 3.7 ) Pub Date : 2018-06-08 00:00:00 , DOI: 10.1021/acs.langmuir.8b00264
Hongmin Ma ₁ , Qi Fan ₁ , Bobo Fan ₁ , Yong Zhang ₁ , Dawei Fan ₁ , Dan Wu ₁ , Qin Wei ₁

Affiliation

开发一种简单但有效的表面修饰方法对于构建生物传感界面非常重要。在这项工作中，已经证明了由肾上腺素的自聚合反应制备的合成后水溶性肾上腺素-黑色素（EPM）作为广泛使用的原位形成的聚多巴胺（PDA）替代品的TiO ₂纳米颗粒表面涂层和光电化学（PEC）生物传感界面的构建。与多巴胺溶液中形成不溶性聚集体相反，自聚合后得到的肾上腺素溶液均匀。使用EPM作为后合成材料可实现TiO ₂的表面涂层使用简单的直接投影法。与广泛使用的原位PDA改性浸涂法相比，基于水溶性后合成EPM的已开发的滴铸法节省了更多时间，避免了大体积溶液的浪费，并且无疑减少了批量生产的时间。批次不一致。市售TiO ₂的简单涂层带有EPM的纳米颗粒由于EPM的电荷转移特性而大大提高了PEC性能。通过将模型生物识别元件（前列腺特异性抗原（PSA）抗体）固定在EPM修饰的氧化铟锡（ITO）光电阳极上，证明了EPM在PEC生物传感界面的构建中的应用。基于PSA抗体的生物学识别能力，获得了具有高选择性和稳定性的灵敏检测PSA。这项工作可能会在其他领域中重新使用合成后黑色素样生物聚合物。

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Formation of Homogeneous Epinephrine-Melanin Solutions to Fabricate Electrodes for Enhanced Photoelectrochemical Biosensing

The development of a simple but effective surface modification method is very important for the construction of biosensing interfaces. In this work, a postsynthetic water-soluble epinephrine-melanin (EPM) prepared from the self-polymerization of epinephrine has been demonstrated as an alternative of the widely used in situ formed polydopamine (PDA) for the surface coating of TiO₂ nanoparticles and the construction of a photoelectrochemical (PEC) biosensing interface. In contrast to the formation of insoluble aggregates in solution for dopamine, a homogeneous solution was obtained for epinephrine after the self-polymerization. The use of EPM as a postsynthetic material enables the surface coating of TiO₂ with the simple drop-casting method. Compared with the widely used dip-coating method for in situ PDA modification, the developed drop-casting method based on the use of water-soluble postsynthetic EPM saves more time, avoids the waste of bulk solution, and undoubtedly decreases the batch-to-batch inconsistencies. The simple coating of commercially available TiO₂ nanoparticles with EPM greatly enhances the PEC performance due to the charge transfer property of EPM. The application of EPM in the construction of the PEC biosensing interface was demonstrated by the immobilization of a model biorecognition element (prostate specific antigen (PSA) antibody) onto EPM modified indium tin oxide (ITO) photoanode. Sensitive detection of PSA with high selectivity and stability was obtained on the basis of the biological recognition ability of PSA antibody. This work may renew the use of postsynthetic melanin-like biopolymers in other fields.

更新日期：2018-06-08

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