当前位置：
X-MOL 学术
›
Anal. Chem.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Simultaneous Detection of Mitochondrial Hydrogen Selenide and Superoxide Anion in HepG2 Cells under Hypoxic Conditions
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-06-08 00:00:00 , DOI: 10.1021/acs.analchem.8b01345 Ranran Cheng 1 , Fanpeng Kong 1 , Lili Tong 1 , Xiaojun Liu 1 , Kehua Xu 1 , Bo Tang 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-06-08 00:00:00 , DOI: 10.1021/acs.analchem.8b01345 Ranran Cheng 1 , Fanpeng Kong 1 , Lili Tong 1 , Xiaojun Liu 1 , Kehua Xu 1 , Bo Tang 1
Affiliation
|
Previous studies proposed that sodium selenite (Na2SeO3) was reduced to hydrogen selenide (H2Se) and that H2Se subsequently reacted with oxygen to generate superoxide anion (O2•–), resulting in tumor cell oxidative stress and apoptosis. However, under the hypoxic conditions of a solid tumor, the anticancer mechanism of sodium selenite remains unclear. To reveal the exact anticancer mechanism of selenite in the real tumor microenvironment, we developed a mitochondria-targeting fluorescent nanosensor, Mito-N-D-MSN, which was fabricated from mesoporous silica nanoparticles (MSNs) loaded with two small-molecule fluorescent probes and a triphenylphosphonium ion as a mitochondria-targeting moiety. With Mito-N-D-MSN, the fluctuations in the contents of mitochondrial hydrogen selenide (H2Se) and superoxide anion (O2•–) in HepG2 cells induced by Na2SeO3 were investigated in detail under normoxic and hypoxic conditions. The results showed that the mitochondrial H2Se content increased gradually, while the O2•– content remained unchanged in HepG2 cells under hypoxic conditions, which indicated that the anticancer mechanism of selenite involves nonoxidative stress in the real tumor microenvironment.
中文翻译：
低氧条件下同时检测HepG2细胞线粒体中的硒化氢和超氧阴离子
先前的研究提出，亚硒酸钠（钠2的SeO 3）还原成硒化氢（H 2 Se）中，并且ħ 2硒随后用氧，以产生反应超氧阴离子（O 2 • -），导致肿瘤细胞氧化应激和凋亡。然而，在实体瘤的低氧条件下，亚硒酸钠的抗癌机制仍不清楚。为了揭示亚硒酸盐在真实肿瘤微环境中的确切抗癌机理，我们开发了一种靶向线粒体的荧光纳米传感器Mito-ND-MSN，该传感器由装有两个小分子荧光探针和三苯基phosph的中孔二氧化硅纳米粒子（MSN）制成。离子作为线粒体靶向部分。利用Mito-ND-MSN，Na 2 SeO 3诱导的HepG2细胞中线粒体硒化氢（H 2 Se）和超氧阴离子（O 2 •–）含量的波动在缺氧和缺氧条件下进行了详细研究。结果表明，在缺氧条件下，HepG2细胞中线粒体H 2 Se含量逐渐增加，而O 2 •–含量保持不变，这表明亚硒酸盐的抗癌机制涉及真实肿瘤微环境中的非氧化应激。
更新日期：2018-06-08
中文翻译：
低氧条件下同时检测HepG2细胞线粒体中的硒化氢和超氧阴离子
先前的研究提出，亚硒酸钠（钠2的SeO 3）还原成硒化氢（H 2 Se）中，并且ħ 2硒随后用氧，以产生反应超氧阴离子（O 2 • -），导致肿瘤细胞氧化应激和凋亡。然而，在实体瘤的低氧条件下，亚硒酸钠的抗癌机制仍不清楚。为了揭示亚硒酸盐在真实肿瘤微环境中的确切抗癌机理，我们开发了一种靶向线粒体的荧光纳米传感器Mito-ND-MSN，该传感器由装有两个小分子荧光探针和三苯基phosph的中孔二氧化硅纳米粒子（MSN）制成。离子作为线粒体靶向部分。利用Mito-ND-MSN，Na 2 SeO 3诱导的HepG2细胞中线粒体硒化氢（H 2 Se）和超氧阴离子（O 2 •–）含量的波动在缺氧和缺氧条件下进行了详细研究。结果表明，在缺氧条件下，HepG2细胞中线粒体H 2 Se含量逐渐增加，而O 2 •–含量保持不变，这表明亚硒酸盐的抗癌机制涉及真实肿瘤微环境中的非氧化应激。
京公网安备 11010802027423号