纳米颗粒的磁性取决于它们在脂质体和细胞上的空间分布<a href="#fn1" title="提供电子补充信息（ESI）：其他实验数据以及有关样品的结构和磁性的信息。参见DOI：10.1039 / c8cp03016b">†</a>,Physical Chemistry Chemical Physics

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纳米颗粒的磁性取决于它们在脂质体和细胞上的空间分布 †
Physical Chemistry Chemical Physics ( IF 2.9 ) Pub Date : 2018-06-05 00:00:00 , DOI: 10.1039/c8cp03016b
Maria Eugenia Fortes Brollo _{1,

2,

3} , Patricia Hernández Flores _{2,

3,

4} , Lucía Gutiérrez _{3,

5,

6} , Christer Johansson _{7,

8,

9} , Domingo Francisco Barber _{2,

3,

4} , María del Puerto Morales _{1,

2,

3}

Affiliation

通过比较纳米粒子空间分布不同的三个系统的磁性，分析了生物系统中磁性纳米粒子的聚集过程。第一个是通过与两种涂层（3-氨基丙基）三甲氧基硅烷（APS）和二巯基琥珀酸（DMSA）共沉淀而合成的14 nm氧化铁纳米颗粒（NPs）。第二种是具有包封的纳米颗粒的脂质体，其取决于NP涂层（附着于脂质体表面或封装在其水体积中的NP）具有不同的构型。最后一个系统由与NP孵育的两个细胞系（Pan02和Jurkat）组成。对液体样品中的动态磁行为（AC）进行了分析，以保持其胶体性质，而对冻干样品进行了准静态（DC）磁测量。交流电测量提供了一种直接的方法，用于确定环境对纳米粒子磁化弛豫的影响。因此，虚构的（随着聚集状态从游离纳米粒子增加到附着或嵌入细胞培养基中脂质体中的那些，当被细胞内化时，χ ′）成分向低频移动。直流磁化曲线显示，在所分析的时间范围内，与生物系统相互作用后，NP并没有降解。然而，无论与位置，孵育时间，细胞系和纳米颗粒涂层如何有关，与细胞接触的纳米颗粒的封闭温度都移到了更高的温度，从而支持了AC磁化率数据。这些结果表明，与细胞接触的简单事实使纳米颗粒以不受控制的方式聚集，这与由于与细胞介质或脂质体内部的接触引起的聚集类型不同。

"点击查看英文标题和摘要"

Magnetic properties of nanoparticles as a function of their spatial distribution on liposomes and cells†

The aggregation processes of magnetic nanoparticles in biosystems are analysed by comparing the magnetic properties of three systems with different spatial distributions of the nanoparticles. The first one is iron oxide nanoparticles (NPs) of 14 nm synthesized by coprecipitation with two coatings, (3-aminopropyl)trimethoxysilane (APS) and dimercaptosuccinic acid (DMSA). The second one is liposomes with encapsulated nanoparticles, which have different configurations depending on the NP coating (NPs attached to the liposome surface or encapsulated in its aqueous volume). The last system consists of two cell lines (Pan02 and Jurkat) incubated with the NPs. Dynamic magnetic behaviour (AC) was analysed in liquid samples, maintaining their colloidal properties, while quasi-static (DC) magnetic measurements were performed on lyophilised samples. AC measurements provide a direct method for determining the effect of the environment on the magnetization relaxation of nanoparticles. Thus, the imaginary (χ′′) component shifts to lower frequencies as the aggregation state increases from free nanoparticles to those attached or embedded into liposomes in cell culture media and more pronounced when internalized by the cells. DC magnetization curves show no degradation of the NPs after interaction with biosystems in the analysed timescale. However, the blocking temperature is shifted to higher temperatures for the nanoparticles in contact with the cells, regardless of the location, the incubation time, the cell line and the nanoparticle coating, supporting AC susceptibility data. These results indicate that the simple fact of being in contact with the cells makes the nanoparticles aggregate in a non-controlled way, which is not the same kind of aggregation caused by the contact with the cell medium nor inside liposomes.

更新日期：2018-06-05

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