<br>一种新型番茄 F-box 蛋白 SlEBF3 参与植物发育和更年期果实成熟过程中乙烯信号的调节,The Plant Journal

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一种新型番茄 F-box 蛋白 SlEBF3 参与植物发育和更年期果实成熟过程中乙烯信号的调节
The Plant Journal ( IF 6.2 ) Pub Date : 2018-06-19 , DOI: 10.1111/tpj.13976
Heng Deng ₁ , Julien Pirrello ₂ , Yao Chen ₁ , Nan Li ₁ , Sihua Zhu ₁ , Ximena Chirinos ₂ , Mondher Bouzayen ₂ , Yongsheng Liu ₁ , Mingchun Liu ₁

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乙烯对于更年期果实成熟至关重要，EIN3 BINDING F-BOX (EBF) 蛋白在拟南芥中通过调节 EIN3/EIL 降解而在介导乙烯反应中发挥核心作用。然而，番茄 EBF 在果实成熟等乙烯依赖性过程中的作用和作用方式仍不清楚。在番茄基因组中鉴定出两个新的 EBF 基因SlEBF3和SlEBF4 ， SlEBF3显示出与成熟相关的表达模式，表明其可能参与控制果实成熟过程中的乙烯反应。 SlEBF3下调的番茄品系未能表现出明显的成熟相关表型，这可能是由于SlEBF家族成员之间的功能冗余所致。相比之下， SlEBF3过表达系表现出与乙烯相关的多效性改变，包括抑制果实成熟、减弱三重反应和延迟花瓣脱落。酵母双杂交系统和双分子荧光互补方法表明，SlEBF3 与所有已知的番茄 SlEIL 蛋白相互作用，并且一致地，SlEBF3 过表达果实中的总 SlEIL 蛋白水平降低，这支持了乙烯敏感性降低和果实成熟缺陷是由乙烯敏感性降低和果实成熟缺陷引起的观点。由于 SlEBF3 介导的 EIL 蛋白降解。此外， SlEBF3 的表达受到 EIL1 通过反馈环的调节，这推测其在调节乙烯信号传导和反应中发挥着作用。总体而言，该研究揭示了新型 EBF 番茄基因在更年期成熟中的作用，从而为调节肉质果实成熟提供了新的靶点。

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A novel tomato F‐box protein, SlEBF3, is involved in tuning ethylene signaling during plant development and climacteric fruit ripening

Ethylene is instrumental to climacteric fruit ripening and EIN3 BINDING F‐BOX (EBF) proteins have been assigned a central role in mediating ethylene responses by regulating EIN3/EIL degradation in Arabidopsis. However, the role and mode of action of tomato EBFs in ethylene‐dependent processes like fruit ripening remains unclear. Two novel EBF genes, SlEBF3 and SlEBF4, were identified in the tomato genome, and SlEBF3 displayed a ripening‐associated expression pattern suggesting its potential involvement in controlling ethylene response during fruit ripening. SlEBF3 downregulated tomato lines failed to show obvious ripening‐related phenotypes likely due to functional redundancy among SlEBF family members. By contrast, SlEBF3 overexpression lines exhibited pleiotropic ethylene‐related alterations, including inhibition of fruit ripening, attenuated triple‐response and delayed petal abscission. Yeast‐two‐hybrid system and bimolecular fluorescence complementation approaches indicated that SlEBF3 interacts with all known tomato SlEIL proteins and, consistently, total SlEIL protein levels were decreased in SlEBF3 overexpression fruits, supporting the idea that the reduced ethylene sensitivity and defects in fruit ripening are due to the SlEBF3‐mediated degradation of EIL proteins. Moreover, SlEBF3 expression is regulated by EIL1 via a feedback loop, which supposes its role in tuning ethylene signaling and responses. Overall, the study reveals the role of a novel EBF tomato gene in climacteric ripening, thus providing a new target for modulating fleshy fruit ripening.

更新日期：2018-06-19

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