当前位置: X-MOL 学术Int. J. Biol. Macromol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Oxyalkylation modification as a promising method for preparing low-melting-point agarose
International Journal of Biological Macromolecules ( IF 7.7 ) Pub Date : 2018-05-24 , DOI: 10.1016/j.ijbiomac.2018.05.171
Na Zhang , Jianglin Wang , Jing Ye , Peng Zhao , Meitian Xiao

In this study, agarose was chemically modified with ethylene oxide, 1,2-epoxypropane and 1,2-epoxybutane, to prepare hydroxyethyl agarose (HEAG), hydroxypropyl agarose (HPAG), and hydroxybutyl agarose (HBAG). The structures and properties of the products were characterized by Fourier transform infrared spectroscopy (FTIR), 1H NMR spectroscopy, X-ray diffractometry (XRD), rheology measurement and gel electrophoresis. The results showed that the gelling temperature (Tg) of HEAG, HPAG, and HBAG were decreased to 28.3 °C, 29.0 °C and 28.7 °C respectively, with the melting temperature (Tm) simultaneously declined to 64.2 °C, 63.8 °C and 63.0 °C, respectively. Meanwhile, the gel strength of HBAG, HEAG, and HPAG were dramatically reduced to 194 g/cm2, 312 g/cm2 and 279 g/cm2, respectively. Furthermore, HEAG and HPAG showed excellent separation efficiency which is in accordance with commercialized low-melting-point (LMP) agarose (Amersco 0815). These results indicated that oxyalkylation is a promising and economical method for the preparation of LMP agarose.



中文翻译:

氧烷基化修饰作为制备低熔点琼脂糖的有前途的方法

在这项研究中,将琼脂糖用环氧乙烷,1,2-环氧丙烷和1,2-环氧丁烷进行化学修饰,以制备羟乙基琼脂糖(HEAG),羟丙基琼脂糖(HPAG)和羟丁基琼脂糖(HBAG)。通过傅里叶变换红外光谱(FTIR),1 H NMR光谱,X射线衍射(XRD),流变学测量和凝胶电泳对产物的结构和性能进行了表征。结果表明,HEAG,HPAG和HBAG的胶凝温度(T g)分别降至28.3°C,29.0°C和28.7°C,熔融温度(T m)同时降至64.2°C,63.8 °C和63.0°C。同时,HBAG,HEAG和HPAG的凝胶强度显着降低至194 g / cm 2分别为312 g / cm 2和279 g / cm 2。此外,HEAG和HPAG表现出优异的分离效率,这与商业化的低熔点(LMP)琼脂糖(Amersco 0815)一致。这些结果表明,氧烷基化是制备LMP琼脂糖的一种有前途且经济的方法。

更新日期:2018-05-24
down
wechat
bug