A new aza-crown ether anthraquinone-coupled chemosensor (2) has been synthesized and employed for the selective fluorescence detection of Mg(II) in the nanomolar range with limited interference by Ca(II) ion. The binding stoichiometry for the chemosensor with Mg(II) is found to be 2:1, and the binding constant was determined to be 2.9×10⁹ M⁻² and 5.1×10⁹ M⁻² in acetonitrile and DMSO, respectively. The chemosensor also shows negligible fluorescence enhancement from other alkali, alkaline earth, and transition metals. Chemosensor 2 has been used in the bioimaging of Mg(II) in embryonic mouse fibroblast NIH3T3 cells at both native concentrations and cells incubated with excess Mg(II). The chemosensor can access Mg(II) ions in both the cytoplasm and nucleus of the cells. The corrected total cell fluorescence is also calculated to confirm the uptake of the chemosensor in living cells.

中文翻译：

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一种简单的含蒽醌荧光团的氮杂冠醚，用于选择性检测活细胞中的Mg（II）

合成了一种新的氮杂-冠醚蒽醌偶联化学传感器（2），并用于在纳摩尔范围内受Ca（II）离子限制的Mg（II）选择性荧光检测。化学传感器与Mg（II）的结合化学计量为2：1，结合常数确定为2.9×10 ⁹ M ^-2和5.1×10 ⁹ M ^-2分别在乙腈和DMSO中 化学传感器还显示出其他碱金属，碱土金属和过渡金属的荧光增强作用可忽略不计。化学传感器2已用于天然浓度和与过量Mg（II）孵育的胚胎小鼠成纤维细胞NIH3T3细胞中Mg（II）的生物成像中。化学传感器可以访问细胞质和细胞核中的Mg（II）离子。还计算校正后的总细胞荧光，以确认化学传感器在活细胞中的摄取。