Fluorescence Polarization Anisotropy in Microdroplets,The Journal of Physical Chemistry Letters

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Fluorescence Polarization Anisotropy in Microdroplets
The Journal of Physical Chemistry Letters ( IF 4.8 ) Pub Date : 2018-05-15 00:00:00 , DOI: 10.1021/acs.jpclett.8b01129
Zhenpeng Zhou ₁ , Xin Yan ₁ , Yin-Hung Lai ₁ , Richard N. Zare ₁

Affiliation

Chemical reactions can be greatly accelerated in microdroplets, but the factors that lead to acceleration are still being elucidated. Using rhodamine 6G (R6G) as a model compound, we studied the density distribution and fluorescence polarization anisotropy of this dye in water-in-oil microdroplets. We found the density of R6G is higher on the surface of the microdroplets, and the ratio of the surface density to that of the center grows with increasing microdroplet radius or with decreasing R6G concentration. The measured fluorescence polarization anisotropy at the surface is almost the same for droplets of different sizes but becomes larger when the concentration is lowered. We also performed three-dimensional simulations by treating R6G⁺ and its associated anion as a dipole of fixed length and magnitude. The simulation results match quite well the experimental measurements, showing that the density distribution and fluorescence polarization anisotropy can be largely explained by a simple electrostatic model.

中文翻译：

微滴中的荧光偏振各向异性

在微滴中，化学反应可以大大加速，但导致加速的因素仍在阐明中。使用罗丹明6G（R6G）作为模型化合物，我们研究了该染料在油包水微滴中的密度分布和荧光偏振各向异性。我们发现，在微滴表面上R6G的密度更高，并且随着微滴半径的增加或R6G浓度的降低，表面密度与中心密度的比值也随之增加。对于不同大小的液滴，在表面处测得的荧光偏振各向异性几乎相同，但当浓度降低时，其变大。我们还通过处理R6G ⁺进行了三维模拟及其相关的阴离子为固定长度和大小的偶极子。仿真结果与实验测量结果非常吻合，表明密度分布和荧光偏振各向异性可以通过简单的静电模型得到很大的解释。

更新日期：2018-05-15

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