There is evidence indicating that curcumin has multiple biological activities, including anti-inflammatory properties. In vitro and in vivo studies demonstrate that curcumin may attenuate inflammation and the connective tissue destruction associated with periodontal disease. Most of these studies use systemic administration, and considering the site-specific nature of periodontal disease and also the poor pharmacodynamic properties of curcumin, we conducted this proof of principle study to assess the biological effect of the local administration of curcumin in a nanoparticle vehicle on experimental periodontal disease. We used 16 rats divided into two groups of 8 animals according to the induction of experimental periodontal disease by bilateral injections of LPS or of the vehicle control directly into the gingival tissues 3×/week for 4 weeks. The same volume of curcumin-loaded nanoparticles or of nanoparticle vehicle was injected into the same sites 2×/week. µCT analysis showed that local administration of curcumin resulted in a complete inhibition of inflammatory bone resorption and in a significant decrease of both osteoclast counts and of the inflammatory infiltrate; as well as a marked attenuation of p38 MAPK and NF-kB activation. We conclude that local administration of curcumin-loaded nanoparticles effectively inhibited inflammation and bone resorption associated with experimental periodontal disease.

中文翻译：

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局部施用姜黄素负载的纳米颗粒可有效抑制与实验性牙周疾病相关的炎症和骨吸收。

有证据表明姜黄素具有多种生物活性，包括抗炎特性。体外和体内研究表明姜黄素可以减轻与牙周疾病有关的炎症和结缔组织破坏。这些研究大多数使用全身给药，考虑到牙周疾病的部位特异性和姜黄素的不良药效学特性，我们进行了这项原理验证研究，以评估纳米颗粒载体中姜黄素的局部给药对糖尿病的生物学作用。实验性牙周病。根据将实验性牙周病通过双边注射LPS或将媒介物对照直接注射入牙龈组织3次/周，诱导4周，将16只大鼠分为8只动物，分为两组。将相同体积的姜黄素负载纳米颗粒或纳米颗粒媒介物每周2次注入相同部位。µCT分析表明，姜黄素的局部给药可完全抑制炎症性骨吸收，并显着减少破骨细胞计数和炎症浸润。以及p38 MAPK和NF-kB激活的明显减弱。我们得出结论，局部施用姜黄素的纳米颗粒可有效抑制与实验性牙周疾病相关的炎症和骨吸收。µCT分析表明，姜黄素的局部给药可完全抑制炎症性骨吸收，并显着减少破骨细胞计数和炎症浸润。以及p38 MAPK和NF-kB激活的明显减弱。我们得出结论，局部施用姜黄素的纳米颗粒可有效抑制与实验性牙周疾病相关的炎症和骨吸收。µCT分析表明，姜黄素的局部给药可完全抑制炎症性骨吸收，并显着减少破骨细胞计数和炎症浸润。以及p38 MAPK和NF-kB激活的明显减弱。我们得出结论，局部施用姜黄素的纳米颗粒可有效抑制与实验性牙周疾病相关的炎症和骨吸收。