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Engineering Adhesive and Antimicrobial Hyaluronic Acid/Elastin-like Polypeptide Hybrid Hydrogels for Tissue Engineering Applications
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2018-04-27 00:00:00 , DOI: 10.1021/acsbiomaterials.8b00408
Ehsan Shirzaei Sani 1 , Roberto Portillo-Lara 1, 2 , Andrew Spencer 1 , Wendy Yu 1 , Benjamin M Geilich 1 , Iman Noshadi 1 , Thomas J Webster 1, 3 , Nasim Annabi 1, 4, 5
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2018-04-27 00:00:00 , DOI: 10.1021/acsbiomaterials.8b00408
Ehsan Shirzaei Sani 1 , Roberto Portillo-Lara 1, 2 , Andrew Spencer 1 , Wendy Yu 1 , Benjamin M Geilich 1 , Iman Noshadi 1 , Thomas J Webster 1, 3 , Nasim Annabi 1, 4, 5
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Hydrogel-based biomaterials have been widely used for tissue engineering applications because of their high water content, swellability, and permeability, which facilitate transport and diffusion of essential nutrients, oxygen, and waste across the scaffold. These characteristics make hydrogels suitable for encapsulating cells and creating a cell supportive environment that promotes tissue regeneration when implanted in vivo. This is particularly important in the context of tissues whose intrinsic regenerative capacity is limited, such as cartilage. However, the clinical translation of hydrogels has been limited by their poor mechanical performance, low adhesive strength, uncontrolled degradation rates, and their susceptibility to bacterial colonization. Here, we introduce an elastic, antimicrobial, and adhesive hydrogel comprised of methacrylated hyaluronic acid (MeHA) and an elastin-like polypeptide (ELP), which can be rapidly photo-cross-linked in situ for the regeneration and repair of different tissues. Hybrid hydrogels with a wide range of physical properties were engineered by varying the concentrations of MeHA and ELP. In addition, standard adhesion tests demonstrated that the MeHA/ELP hydrogels exhibited higher adhesive strength to the tissue than commercially available tissue adhesives. MeHA/ELP hydrogels were then rendered antimicrobial through the incorporation of zinc oxide (ZnO) nanoparticles, and were shown to significantly inhibit the growth of methicillin-resistant Staphylococcus aureus (MRSA), as compared to controls. Furthermore, the composite adhesive hydrogels supported in vitro mammalian cellular growth, spreading, and proliferation. In addition, in vivo subcutaneous implantation demonstrated that MeHA/ELP hydrogels did not elicit any significant inflammatory response, and could be efficiently biodegraded while promoting the integration of new autologous tissue. In summary, we demonstrated for the first time that MeHA/ELP-ZnO hydrogel can be used as an adhesive and antimicrobial biomaterial for tissue engineering applications, because of its highly tunable physical characteristics, as well as remarkable adhesive and antimicrobial properties.
中文翻译:
用于组织工程应用的工程粘合剂和抗菌透明质酸/类弹性蛋白多肽杂化水凝胶
基于水凝胶的生物材料因其高含水量、膨胀性和渗透性而被广泛用于组织工程应用,这有利于必需营养物质、氧气和废物在支架上的运输和扩散。这些特性使水凝胶适合封装细胞并创造细胞支持环境,在植入体内时促进组织再生。这对于固有再生能力有限的组织(例如软骨)尤其重要。然而,水凝胶的临床转化因其较差的机械性能、低粘合强度、不受控制的降解率以及对细菌定植的敏感性而受到限制。在这里,我们介绍了一种由甲基丙烯酸透明质酸(MeHA)和类弹性蛋白多肽(ELP)组成的弹性、抗菌和粘合水凝胶,它可以在原位快速光交联,用于不同组织的再生和修复。通过改变 MeHA 和 ELP 的浓度,设计出具有多种物理特性的混合水凝胶。此外,标准粘附测试表明,MeHA/ELP 水凝胶对组织的粘附强度比市售的组织粘合剂更高。 MeHA/ELP 水凝胶通过掺入氧化锌 (ZnO) 纳米颗粒而具有抗菌作用,与对照相比,MeHA/ELP 水凝胶可显着抑制耐甲氧西林金黄色葡萄球菌(MRSA) 的生长。此外,复合粘合水凝胶支持体外哺乳动物细胞的生长、扩散和增殖。 此外,体内皮下植入表明MeHA/ELP水凝胶不会引起任何明显的炎症反应,并且可以有效地生物降解,同时促进新的自体组织的整合。总之,我们首次证明MeHA/ELP-ZnO水凝胶由于其高度可调的物理特性以及卓越的粘合和抗菌性能,可用作组织工程应用的粘合和抗菌生物材料。
更新日期:2018-04-27
中文翻译:
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用于组织工程应用的工程粘合剂和抗菌透明质酸/类弹性蛋白多肽杂化水凝胶
基于水凝胶的生物材料因其高含水量、膨胀性和渗透性而被广泛用于组织工程应用,这有利于必需营养物质、氧气和废物在支架上的运输和扩散。这些特性使水凝胶适合封装细胞并创造细胞支持环境,在植入体内时促进组织再生。这对于固有再生能力有限的组织(例如软骨)尤其重要。然而,水凝胶的临床转化因其较差的机械性能、低粘合强度、不受控制的降解率以及对细菌定植的敏感性而受到限制。在这里,我们介绍了一种由甲基丙烯酸透明质酸(MeHA)和类弹性蛋白多肽(ELP)组成的弹性、抗菌和粘合水凝胶,它可以在原位快速光交联,用于不同组织的再生和修复。通过改变 MeHA 和 ELP 的浓度,设计出具有多种物理特性的混合水凝胶。此外,标准粘附测试表明,MeHA/ELP 水凝胶对组织的粘附强度比市售的组织粘合剂更高。 MeHA/ELP 水凝胶通过掺入氧化锌 (ZnO) 纳米颗粒而具有抗菌作用,与对照相比,MeHA/ELP 水凝胶可显着抑制耐甲氧西林金黄色葡萄球菌(MRSA) 的生长。此外,复合粘合水凝胶支持体外哺乳动物细胞的生长、扩散和增殖。 此外,体内皮下植入表明MeHA/ELP水凝胶不会引起任何明显的炎症反应,并且可以有效地生物降解,同时促进新的自体组织的整合。总之,我们首次证明MeHA/ELP-ZnO水凝胶由于其高度可调的物理特性以及卓越的粘合和抗菌性能,可用作组织工程应用的粘合和抗菌生物材料。