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Accelerating the Peroxidase-Like Activity of Gold Nanoclusters at Neutral pH for Colorimetric Detection of Heparin and Heparinase Activity
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-04-26 00:00:00 , DOI: 10.1021/acs.analchem.8b00885 Lianzhe Hu 1 , Hong Liao 1 , Lingyan Feng 2 , Min Wang 3 , Wensheng Fu 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-04-26 00:00:00 , DOI: 10.1021/acs.analchem.8b00885 Lianzhe Hu 1 , Hong Liao 1 , Lingyan Feng 2 , Min Wang 3 , Wensheng Fu 1
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The peroxidase-like catalytic activity of gold nanoclusters (Au-NCs) is quite low around physiological pH, which greatly limits their biological applications. Herein, we found heparin can greatly accelerate the peroxidase-like activity of Au-NCs at neutral pH. The catalytic activity of Au-NCs toward the peroxidase substrate 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation by H2O2 was 25-fold increased in the presence of heparin at pH 7. The addition of heparin not only accelerated the initial catalytic rate of Au-NCs but also prevented the Au-NCs from catalyst deactivation. This allows the sensitive colorimetric detection of heparin at neutral pH. In the presence of heparinase, heparin was hydrolyzed into small fragments, weakening the enhancement effect of catalytic activity. On the basis of this phenomenon, the colorimetric determination of heparinase in the range from 0.1 to 3 μg·mL–1 was developed with a detection limit of 0.06 μg·mL–1. Finally, the detection of heparin and heparinase activity in diluted serum samples was also demonstrated.
中文翻译：
在中性pH值下加速金纳米簇的过氧化物酶样活性，用于比色检测肝素和肝素酶活性
金纳米簇（Au-NCs）的过氧化物酶样催化活性在生理pH值附近非常低，这极大地限制了它们的生物学应用。在这里，我们发现肝素可以在中性pH值下大大促进Au-NCs的过氧化物酶样活性。Au-NCs对H 2 O 2对过氧化物酶底物3,3'，5,5'-四甲基联苯胺（TMB）的催化活性在pH 7的肝素存在下，α-NCs的催化活性增加了25倍。添加肝素不仅加速了Au-NCs的初始催化速率，而且还阻止了Au-NCs的催化剂失活。这允许在中性pH值下灵敏地比色检测肝素。在肝素酶的存在下，肝素被水解成小碎片，削弱了催化活性的增强作用。基于此现象，开发了比色法测定肝素酶的范围为0.1至3μg·mL –1，检测极限为0.06μg·mL –1。最后，还证明了在稀释的血清样品中检测肝素和肝素酶活性。
更新日期：2018-04-26
中文翻译：
在中性pH值下加速金纳米簇的过氧化物酶样活性，用于比色检测肝素和肝素酶活性
金纳米簇（Au-NCs）的过氧化物酶样催化活性在生理pH值附近非常低，这极大地限制了它们的生物学应用。在这里，我们发现肝素可以在中性pH值下大大促进Au-NCs的过氧化物酶样活性。Au-NCs对H 2 O 2对过氧化物酶底物3,3'，5,5'-四甲基联苯胺（TMB）的催化活性在pH 7的肝素存在下，α-NCs的催化活性增加了25倍。添加肝素不仅加速了Au-NCs的初始催化速率，而且还阻止了Au-NCs的催化剂失活。这允许在中性pH值下灵敏地比色检测肝素。在肝素酶的存在下，肝素被水解成小碎片，削弱了催化活性的增强作用。基于此现象，开发了比色法测定肝素酶的范围为0.1至3μg·mL –1，检测极限为0.06μg·mL –1。最后，还证明了在稀释的血清样品中检测肝素和肝素酶活性。
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