This work reports new results about the solvent-free enzymatic production of ethylene glycol monostearate, an industrially important emollient ester conventionally produced by a chemical route. For this purpose, reactions were carried out using two commercial immobilized lipases, evaluating the effect of temperature, agitation, and enzyme concentration on the reaction conversion. It was shown that, for most tested conditions, reaction conversions around 100% were achieved. The reuse of the enzyme was also evaluated with satisfactory results up to four enzyme cycles. System scale-up by 9-fold allowed reaching conversions of up to 99%. Chemical analyzes showed that the product obtained through enzymatic synthesis presented very good characteristics, which makes the enzymatic esterification process employed in this work an interesting and promising route for possible application at industrial scale.

中文翻译：

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通过酶促酯化无溶剂生产乙二醇单硬脂酸酯

这项工作报告了有关无溶剂酶法生产乙二醇单硬脂酸酯的新结果，乙二醇单硬脂酸酯是常规上通过化学途径生产的工业上重要的润肤酯。为此目的，使用两种市售的固定化脂肪酶进行反应，评估温度，搅拌和酶浓度对反应转化率的影响。结果表明，在大多数测试条件下，反应转化率均达到约100％。还评估了酶的重复使用，直到四个酶循环，结果令人满意。系统放大9倍，可以实现高达99％的转化率。化学分析表明，通过酶促合成获得的产物具有很好的特性，