Shear Stress-Enhanced Internalization of Cell Membrane Proteins Indicated by a Hairpin-Type DNA Probe,Analytical Chemistry

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Shear Stress-Enhanced Internalization of Cell Membrane Proteins Indicated by a Hairpin-Type DNA Probe
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-04-02 00:00:00 , DOI: 10.1021/acs.analchem.8b00755
Ziyi He ₁ , Wanling Zhang ₁ , Sifeng Mao ₁ , Nan Li ₁ , Haifang Li ₁ , Jin-Ming Lin ₁

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Shear stress is an important mechanical stimulus that plays a critical role in modulating cell functions. In this study, we investigated the regulating effects of shear stress on the internalization of cell membrane proteins in a microfluidic chip. A hairpin-type DNA probe was developed and indiscriminately anchored to the cell surface, acting as an indicator for the membrane proteins. When cells were exposed to shear stress generated from fluid cell medium containing external proteins, strong fluorescence was emanated from intracellular regions. With intensive investigation, results revealed that shear stress could enhance the specific cell endocytosis pathway and promote membrane protein internalization. This process was indicated by the enhanced intracellular fluorescence, generated from the internalized and mitochondria accumulated DNA probes. This study not only uncovered new cellular mechanotransduction mechanisms but also provided a versatile method that enabled in situ and dynamic indication of cell responses to mechanical stimuli.

中文翻译：

发夹型DNA探针表明，剪切应力增强了细胞膜蛋白的内在化。

剪应力是重要的机械刺激，在调节细胞功能中起关键作用。在这项研究中，我们研究了剪切应力对微流控芯片中细胞膜蛋白内在化的调节作用。形成了发夹型DNA探针，该探针无差别地锚定在细胞表面，充当膜蛋白的指示剂。当细胞暴露于由含有外部蛋白质的液体细胞培养基产生的切应力时，从细胞内区域发出强烈的荧光。经过深入的研究，结果表明剪切应力可以增强特定的细胞内吞途径并促进膜蛋白的内在化。该过程由增强的细胞内荧光指示，该荧光是由内部化和线粒体积累的DNA探针产生的。

更新日期：2018-04-02

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