Stem Cell Defect in UBC-GFP Mice Facilitates Engraftment of Lymphoid-Primed Hematopoietic Stem Cells,STEM CELLS

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Stem Cell Defect in UBC-GFP Mice Facilitates Engraftment of Lymphoid-Primed Hematopoietic Stem Cells
STEM CELLS ( IF 4.0 ) Pub Date : 2018-04-15 , DOI: 10.1002/stem.2828
Kateřina Faltusová ₁ , Katarína Szikszai ₁ , Martin Molík ₁ , Jana Linhartová ₁ , Petr Páral ₁ , Luděk Šefc ₁ , Filipp Savvulidi ₁ , Emanuel Nečas _{1,

2}

Affiliation

Transgenic mice expressing green fluorescent protein (GFP) are useful in transplantation experiments. When we used ubiquitin‐GFP (UBC‐GFP) transgenic mice to study the availability of niches for transplanted hematopoietic stem and progenitor cells, the results were strikingly different from the corresponding experiments that used congenic mice polymorphic in the CD45 antigen. Analysis of these unexpected results revealed that the hematopoiesis of UBC‐GFP mice was outcompeted by the hematopoiesis of wild‐type (WT) mice. Importantly, UBC‐GFP mice engrafted the transplanted bone marrow of WT mice without conditioning. There was a significant bias toward lymphopoiesis in the WT branch of chimeric UBC‐GFP/WT hematopoiesis. A fraction of immature Sca‐1+ cells in the spleen of UBC‐GFP mice expressed GFP at a very high level. The chimeric hematopoiesis was stable in the long term and also after transplantation to secondary recipient mice. The article thus identifies a specific defect in the hematopoiesis of UBC‐GFP transgenic mice that compromises the lymphoid‐primed hematopoietic stem cells in the bone marrow and spleen. Stem Cells 2018;36:1237–1248

中文翻译：

UBC-GFP 小鼠的干细胞缺陷促进淋巴样造血干细胞的植入

表达绿色荧光蛋白 (GFP) 的转基因小鼠可用于移植实验。当我们使用泛素-GFP (UBC-GFP) 转基因小鼠来研究移植造血干细胞和祖细胞的生态位的可用性时，结果与使用 CD45 抗原多态性的同类小鼠的相应实验截然不同。对这些意外结果的分析表明，UBC-GFP 小鼠的造血能力被野生型 (WT) 小鼠的造血能力所超越。重要的是，UBC-GFP 小鼠在没有调节的情况下移植了 WT 小鼠的移植骨髓。在嵌合 UBC-GFP/WT 造血的 WT 分支中存在显着的淋巴细胞生成偏向。UBC-GFP 小鼠脾脏中的一小部分未成熟的 Sca-1+ 细胞以非常高的水平表达 GFP。嵌合造血是长期稳定的，并且在移植到二级受体小鼠后也是稳定的。因此，该文章确定了 UBC-GFP 转基因小鼠造血功能的特定缺陷，该缺陷会损害骨髓和脾脏中的淋巴样造血干细胞。干细胞 2018；36：1237–1248

更新日期：2018-04-15

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