Abstract The objective of this study was to investigate the physicochemical and interfacial rheological properties of ovalbumin (OVA)-carboxymethylcellulose (CMC) nanoparticles. The OVA/CMCs nanoparticles were prepared by heating (90 °C, 30 min) the electrostatic self-assembly complexes between OVA and CMC of different charge density (CMC 0.7 and CMC 1.2) at pH 4.4. The results showed that the OVA/CMC 0.7 nanoparticles exhibited larger size and lower surface net potential than OVA/CMC 1.2 nanoparticles. Atomic force microscopy (AFM) imaging and ultra-small angle X-ray scattering (USAXS) results suggested that the shape of the particles was approximately spherical, and the structure of OVA/CMC 1.2 nanoparticles was more compact than that of OVA/CMC 0.7. The pyrene fluorescent probe indicated that the OVA/CMC 1.2 nanoparticles had a stronger hydrophobicity than OVA/CMC 0.7 nanoparticles in the range of pH 4–7. As the pH and the ionic strength increased, the average diameter of OVA/CMC nanoparticles would increase (

中文翻译：

---

卵清蛋白-羧甲基纤维素复合物热处理制备纳米颗粒的表征和界面流变特性

摘要 本研究的目的是研究卵清蛋白 (OVA)-羧甲基纤维素 (CMC) 纳米粒子的物理化学和界面流变特性。OVA/CMCs 纳米粒子是通过在 pH 4.4 下加热（90°C，30 分钟）OVA 和不同电荷密度（CMC 0.7 和 CMC 1.2）的 CMC 之间的静电自组装复合物来制备的。结果表明，与 OVA/CMC 1.2 纳米颗粒相比，OVA/CMC 0.7 纳米颗粒表现出更大的尺寸和更低的表面净电位。原子力显微镜 (AFM) 成像和超小角 X 射线散射 (USAXS) 结果表明粒子的形状近似球形，并且 OVA/CMC 1.2 纳米粒子的结构比 OVA/CMC 0.7 更紧凑. 芘荧光探针表明 OVA/CMC 1. 在 pH 4-7 范围内，2 纳米颗粒比 OVA/CMC 0.7 纳米颗粒具有更强的疏水性。随着 pH 值和离子强度的增加，OVA/CMC 纳米粒子的平均直径会增加（