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Impact of primer dimers and self-amplifying hairpins on reverse transcription loop-mediated isothermal amplification detection of viral RNA
The Analyst Pub Date : 2018-03-27 , DOI: 10.1039/c7an01897e Robert J. Meagher 1, 2, 3, 4 , Aashish Priye 1, 2, 3, 4 , Yooli K. Light 1, 3, 4, 5 , Cheng Huang 4, 6, 7, 8 , Eryu Wang 4, 6, 8, 9, 10
中文翻译：
引物二聚体和自扩增发夹对逆转录环介导的病毒RNA等温扩增检测的影响
更新日期：2018-03-27
The Analyst Pub Date : 2018-03-27 , DOI: 10.1039/c7an01897e Robert J. Meagher 1, 2, 3, 4 , Aashish Priye 1, 2, 3, 4 , Yooli K. Light 1, 3, 4, 5 , Cheng Huang 4, 6, 7, 8 , Eryu Wang 4, 6, 8, 9, 10
Affiliation
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Bumping the priming sites for RT-LAMP based on thermodynamic predictions can dramatically reduce non-specific background amplification.
中文翻译：
引物二聚体和自扩增发夹对逆转录环介导的病毒RNA等温扩增检测的影响
根据热力学预测碰撞 RT-LAMP 的引发位点可以显着减少非特异性背景扩增。
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