Analysis of Metabolomic Changes in Mesenchymal Stem Cells on Treatment with Desferrioxamine as a Hypoxia Mimetic Compared to Hypoxic Conditions,STEM CELLS

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Analysis of Metabolomic Changes in Mesenchymal Stem Cells on Treatment with Desferrioxamine as a Hypoxia Mimetic Compared to Hypoxic Conditions
STEM CELLS ( IF 4.0 ) Pub Date : 2018-04-10 , DOI: 10.1002/stem.2826
Koichi Fujisawa _{1,

2} , Taro Takami ₂ , Sae Okada ₂ , Kazusa Hara _{2,

3} , Toshihiko Matsumoto ₂ , Naoki Yamamoto ₂ , Takahiro Yamasaki ₄ , Isao Sakaida _{1,

2}

Affiliation

Mesenchymal stem cells (MSCs) are commonly used in regenerative medicine, but their therapeutic effects vary depending on the culture environment. Hypoxic culturing can be used to maintain stem cells in an undifferentiated state, but is expensive and difficult to perform. The aim of this study was to determine the effectiveness of desferrioxamine (DFO), a hypoxia‐mimetic reagent, as an alternative to hypoxic culturing by analyzing metabolic changes in MSCs under hypoxic conditions compared with changes induced by DFO. Low concentrations of DFO reduced mitochondrial activity and apoptosis. Therefore, low concentrations of DFO may be useful for MSC preconditioning. Metabolome analysis showed that both hypoxic treatment and DFO administration exhibited similar metabolite patterns except purine, pyrimidine, and tricarboxylic acid cycle (TCA) cycle related metabolites. Therefore, the use of DFO at low concentrations is a potential substitute for hypoxic culturing. These findings may form the foundation for the development of future regenerative therapies using MSCs. Stem Cells 2018;36:1226–1236

中文翻译：

与缺氧条件相比，用去铁胺作为缺氧模拟物处理间充质干细胞的代谢组学变化分析

间充质干细胞 (MSCs) 常用于再生医学，但其治疗效果因培养环境而异。缺氧培养可用于将干细胞维持在未分化状态，但成本高且难以实施。本研究的目的是通过分析缺氧条件下 MSCs 的代谢变化与 DFO 诱导的变化相比，确定去铁胺（DFO）（一种缺氧模拟试剂）作为缺氧培养替代方案的有效性。低浓度的 DFO 会降低线粒体活性和细胞凋亡。因此，低浓度的 DFO 可用于 MSC 预处理。代谢组分析表明，除了嘌呤、嘧啶、和三羧酸循环 (TCA) 循环相关的代谢物。因此，在低浓度下使用 DFO 是缺氧培养的潜在替代品。这些发现可能为使用 MSC 开发未来的再生疗法奠定基础。干细胞 2018；36：1226–1236

更新日期：2018-04-10

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