Deep sequencing was used to determine complete nucleotide sequences of echovirus 11 (EV11) strains isolated from a chronically infected patient with CVID as well as from cases of acute enterovirus infection. Phylogenetic analysis showed that EV11 strains that circulated in Israel in 1980-90s could be divided into four clades. EV11 strains isolated from a chronically infected individual belonged to one of the four clades and over a period of 4 years accumulated mutations at a relatively constant rate. Extrapolation of mutations accumulation curve into the past suggested that the individual was infected with circulating EV11 in the first half of 1990s. Genomic regions coding for individual viral proteins did not appear to be under strong selective pressure except for protease 3C that was remarkably conserved. This may suggest its important role in maintaining persistent infection.

深度测序用于确定从CVID慢性感染患者以及急性肠病毒感染病例中分离出的回声病毒11（EV11）菌株的完整核苷酸序列。系统发育分析表明，1980-90年代在以色列传播的EV11病毒株可分为四个进化枝。从慢性感染者中分离出的EV11菌株属于四个进化枝之一，并在4年的时间内以相对恒定的速率积累了突变。根据过去积累的突变积累曲线推断，该个体在1990年代上半年感染了循环中的EV11。编码单个病毒蛋白的基因组区域似乎没有处于强烈的选择性压力下，除了蛋白酶3C显着保守。