MAP3Kinase-dependent SnRK2-kinase activation is required for abscisic acid signal transduction and rapid osmotic stress response.,Nature Communications

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MAP3Kinase-dependent SnRK2-kinase activation is required for abscisic acid signal transduction and rapid osmotic stress response.
Nature Communications ( IF 14.7 ) Pub Date : 2020-01-02 , DOI: 10.1038/s41467-019-13875-y
Yohei Takahashi ₁ , Jingbo Zhang _{1,

2} , Po-Kai Hsu ₁ , Paulo H O Ceciliato ₁ , Li Zhang ₁ , Guillaume Dubeaux ₁ , Shintaro Munemasa ₃ , Chennan Ge ₁ , Yunde Zhao ₁ , Felix Hauser ₁ , Julian I Schroeder ₁

Affiliation

Abiotic stresses, including drought and salinity, trigger a complex osmotic-stress and abscisic acid (ABA) signal transduction network. The core ABA signalling components are snf1-related protein kinase2s (SnRK2s), which are activated by ABA-triggered inhibition of type-2C protein-phosphatases (PP2Cs). SnRK2 kinases are also activated by a rapid, largely unknown, ABA-independent osmotic-stress signalling pathway. Here, through a combination of a redundancy-circumventing genetic screen and biochemical analyses, we have identified functionally-redundant MAPKK-kinases (M3Ks) that are necessary for activation of SnRK2 kinases. These M3Ks phosphorylate a specific SnRK2/OST1 site, which is indispensable for ABA-induced reactivation of PP2C-dephosphorylated SnRK2 kinases. ABA-triggered SnRK2 activation, transcription factor phosphorylation and SLAC1 activation require these M3Ks in vitro and in plants. M3K triple knock-out plants show reduced ABA sensitivity and strongly impaired rapid osmotic-stress-induced SnRK2 activation. These findings demonstrate that this M3K clade is required for ABA- and osmotic-stress-activation of SnRK2 kinases, enabling robust ABA and osmotic stress signal transduction.

中文翻译：

脱落酸信号转导和快速渗透应激反应需要MAP3Kinase依赖SnRK2-激酶激活。

非生物胁迫（包括干旱和盐碱化）引发复杂的渗透胁迫和脱落酸（ABA）信号转导网络。核心的ABA信号转导成分是snf1相关的蛋白激酶2（SnRK2），其通过ABA触发的2C型蛋白磷酸酶（PP2C）的抑制而被激活。SnRK2激酶也可以通过快速的，很大程度上未知的，与ABA无关的渗透压信号通路激活。在这里，通过冗余规避遗传筛选和生化分析的组合，我们已经确定了功能冗余的MAPKK激酶（M3K），这是激活SnRK2激酶所必需的。这些M3K磷酸化一个特定的SnRK2 / OST1位点，这对于ABA诱导的PP2C去磷酸化的SnRK2激酶的激活是必不可少的。ABA触发的SnRK2激活，转录因子磷酸化和SLAC1激活需要体外和植物中的这些M3K。M3K三联基因敲除植物显示降低的ABA敏感性，并严重损害了快速渗透胁迫诱导的SnRK2活化。这些发现表明，此M3K进化枝是SnRK2激酶的ABA和渗透胁迫激活所必需的，从而能够实现强大的ABA和渗透胁迫信号转导。

更新日期：2020-01-02

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