In the current paper, methyl-12-hydroxyoctadec-9-enoate was synthesized by biocatalytic transesterification of castor oil with methanol in presence of two different indigenously immobilized lipase preparations. Aiming for optimum yield (94% methyl-12-hydroxyoctadec-9-enoate), the batch reaction was conducted to evaluate the influence of reaction time, oil to methanol ratio, and water content. The atom economy (91.67%) and E-factor (0.297) for the studied reaction was uncovered, as well as an analysis of the adherence of the twelve principles of green chemistry was summarized. The green credentials were assessed using an efficient tool in strategic planning, a strengths-weaknesses-opportunities-threats (SWOT) analysis. The operational stability of immobilized lipase reactor column (60 °C) was determined for a prolonged period (>300 h) with the intermittent water addition to maintaining live enzyme. Furthermore, the purification of methyl-12-hydroxyoctadec-9-enoate using liquid-liquid extraction aided purity of 98%, confirmed by HPLC and GC-MS analysis.

在当前的论文中，在两种不同的本地固定脂肪酶制剂的存在下，通过蓖麻油与甲醇的生物催化酯交换反应合成了12-羟基十八烷基-9-烯酸甲酯。为了获得最佳收率（94％的12-羟基十八烷基9-烯酸甲酯），进行间歇反应以评估反应时间，油/甲醇比和水含量的影响。揭示了所研究反应的原子经济性（91.67％）和电子因子（0.297），并对绿色化学的十二个原理的依从性进行了分析。使用有效的战略规划工具，优势-劣势-机会-威胁（SWOT）分析对绿色证书进行了评估。确定了固定的脂肪酶反应器柱（60°C）长时间（> 300 h）用间歇水除以维持活性酶。此外，通过液-液萃取纯化的12-羟基十八烷基-9-烯酸甲酯的纯度为98％，这已通过HPLC和GC-MS分析证实。