介孔二氧化钛涂层对铜绿假单胞菌生物膜的抗生物膜作用。,Journal of Photochemistry and Photobiology B: Biology

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介孔二氧化钛涂层对铜绿假单胞菌生物膜的抗生物膜作用。
Journal of Photochemistry and Photobiology B: Biology ( IF 3.9 ) Pub Date : 2019-12-24 , DOI: 10.1016/j.jphotobiol.2019.111762
Magdalena Pezzoni ₁ , Paolo N Catalano ₂ , Diana C Delgado ₂ , Ramón A Pizarro ₁ , Martín G Bellino ₂ , Cristina S Costa ₁

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已经提出了通过紫外线-A（UVA）辐射活化光催化二氧化钛作为抵抗细菌的好方法。溶液中或固定在表面上的二氧化钛粉末对多种微生物具有出色的UVA辅助杀灭特性。但是，这些特性在铜绿假单胞菌的生物膜中无法得到证实，铜绿假单胞菌是一种具有抵抗力的机会性人类病原体，可在免疫功能低下或烧伤创面或囊性纤维化患者中引起严重的并发症。铜绿假单胞菌生物膜对健康和工业具有有害影响，造成严重的经济损失。在这项研究中，通过使用通过溶胶-凝胶和蒸发诱导的自组装获得的不同涂层，研究了二氧化钛光催化控制铜绿假单胞菌生物膜的效果。生物膜在具有不同孔径的非中孔和中孔二氧化钛表面上生长，这是基于使用表面活性剂Brij-58和Pluronics-F127实现的。另外，测定了二氧化钛的两种结构形式：无定形和锐钛矿。这些涂层不仅抑制生物膜的形成，而且还显着增强了UVA对铜绿假单胞菌生物膜的杀菌作用。关于总的抗菌效果，最有效的表面是介孔的Brij-58模板锐钛酶薄膜，与对照生物薄膜相比，该薄膜将活菌数量减少了约5个数量级，证明了这种方法作为消毒系统的功效。测定了二氧化钛的两种结构形式：无定形和锐钛矿。这些涂层不仅抑制生物膜的形成，而且还显着增强了UVA对铜绿假单胞菌生物膜的杀菌作用。关于总的抗菌效果，最有效的表面是介孔的Brij-58模板锐钛酶薄膜，与对照生物薄膜相比，该薄膜将活菌数量减少了约5个数量级，证明了这种方法作为消毒系统的功效。测定了二氧化钛的两种结构形式：无定形和锐钛矿。这些涂层不仅抑制生物膜的形成，而且还显着增强了UVA对铜绿假单胞菌生物膜的杀菌作用。关于总的抗菌效果，最有效的表面是介孔的Brij-58模板锐钛酶薄膜，与对照生物薄膜相比，该薄膜将活菌数量减少了约5个数量级，证明了这种方法作为消毒系统的功效。

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Antibiofilm effect of mesoporous titania coatings on Pseudomonas aeruginosa biofilms.

Activation of photocatalytic titania by ultraviolet-A (UVA) radiation has been proposed as a good approach for combating bacteria. Titania powder, in solution or immobilized on a surface, has excellent UVA-assisted killing properties on several microorganisms. However, these properties could not be demonstrated in biofilms of Pseudomonas aeruginosa, a resistant opportunistic human pathogen that can cause severe complications in patients who are immunocompromised or have burn wounds or cystic fibrosis. P. aeruginosa biofilms have detrimental effects on health and industry, causing serious economic damage. In this study, the effect of titania photocatalysis for controlling P. aeruginosa biofilms was investigated by employing different coatings obtained through sol-gel and evaporation-induced self-assembly. Biofilms were grown on non-mesoporous and mesoporous titania surfaces with different pore sizes, which were achieved based on the use of surfactants Brij-58 and Pluronics-F127. In addition, two structural forms of titania were assayed: amorphous and anatase. As well as inhibiting biofilm formation, these coatings significantly enhanced the bactericidal effect of UVA on P. aeruginosa biofilms. The most efficient surface with regard to total antibacterial effect was the mesoporous Brij-58-templated anatase film, which, compared to control biofilms, decreased the number of viable bacteria by about 5 orders, demonstrating the efficacy of this methodology as a disinfection system.

更新日期：2019-12-25

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