In this work, two polymeric membrane separators (a proton exchange membrane (PEM), Nafion, and an anion-exchange membrane (AEM), 1,4-diazabicyclo[2.2.2]octane (DABCO)-functionalized PSEBS) deployed in microbial fuel cells (MFCs) are comparatively assessed. The performances of MFCs according to membrane type were evaluated by biological and electrochemical techniques, employing metagenomics, electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). It was found that the anodic biofilms of MFCs, irrespective of the type of membrane, were dominated by Geobacter sulfurreducens (37 and 50 % for AEM-MFC and PEM-MFC, respectively), a well-known electrochemically active species. Furthermore, the AEM-MFC reflected a significantly lower internal resistance (145 Ω) compared to PEM-MFC (339 Ω) and produced higher maximal current densities and energy yields at all substrate (acetate) concentrations, as follows: 400 vs. 285 mA m^-2 (5 mM acetate); 360 vs. 320 mA m^-2 (10 mM acetate), 305 vs. 235 mA m^-2 (15 mM acetate) and 238 vs.132 kJ m^-2 g_COD (5 mM acetate), 161 vs. 128 kJ m^-2 g_COD (10 mM acetate), 114 vs. 59 kJ m^-2 g_COD (15 mM acetate) respectively. The CV measurements implied diffusion limitations in the MFCs, which were supported by EIS. In addition, the PEM and AEM characterizations revealed that in both cases, the ion exchange capacity, ionic conductivity and oxygen mass transport features were altered considerably over the 39 days during which the MFCs operated.

在这项工作中，部署在微生物中的两个聚合物膜分离器（质子交换膜（PEM），Nafion和阴离子交换膜（AEM），1,4-二氮杂双环[2.2.2]辛烷（DABCO）功能化的PSEBS）对燃料电池（MFC）进行了比较评估。通过使用宏基因组学，电化学阻抗谱（EIS）和循环伏安法（CV）的生物学和电化学技术评估了根据膜类型的MFC性能。已发现，不论膜的类型如何，MFCs的阳极生物膜均由还原性土壤杆菌 （分别为AEM-MFC和PEM-MFC的37％和50％）占主导地位 ， 众所周知的电化学活性物质。此外，与PEM-MFC（339Ω）相比，AEM-MFC的内电阻（145Ω）明显更低，并且在所有底物（乙酸盐）浓度下产生的最大最大电流密度和能量产生分别如下：400 vs. 285 mA m ^-2（5 mM乙酸盐）; 360 vs.320 mA m ^-2（10 mM乙酸盐），305 vs.235 mA m ^-2（15 mM乙酸盐）和238 vs.132 kJ m ^-2 g _COD（5 mM乙酸盐），161 vs. 128 kJ m ^-2 g _COD（10 mM乙酸盐），114 vs. 59 kJ m ^-2 g _COD（分别为15 mM醋酸盐）。CV测量暗示了MFC中的扩散限制，EIS支持了这一点。此外，PEM和AEM的特征表明，在这两种情况下，MFCs运行的39天中，离子交换容量，离子电导率和氧气质量传输特征均发生了显着变化。