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Immunity-related genes and signaling pathways under hypoxic stresses in Haliotis diversicolor: a transcriptome analysis.
Scientific Reports ( IF 3.8 ) Pub Date : 2019-12-24 , DOI: 10.1038/s41598-019-56150-2 Yulong Sun 1, 2 , Xin Zhang 1, 2 , Yilei Wang 3 , Robert Day 4 , Huiping Yang 5 , Ziping Zhang 1, 3
Scientific Reports ( IF 3.8 ) Pub Date : 2019-12-24 , DOI: 10.1038/s41598-019-56150-2 Yulong Sun 1, 2 , Xin Zhang 1, 2 , Yilei Wang 3 , Robert Day 4 , Huiping Yang 5 , Ziping Zhang 1, 3
Affiliation
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Due to increased temperatures and aquaculture density, thermal and hypoxia stresses have become serious problems for the aquaculture of abalone Haliotis diversicolor. Stresses lead to immunosuppression, which can cause severe negative impacts on aquaculture farms. To study the mechanism of immunosuppression after hypoxia stress and bacterial challenge, transcriptomes of H. diversicolor hemocytes involved in immunity were profiled. A total of 307,395,572 clean reads were generated and assembled into 99,774 unigenes. KEGG analysis indicated that 225 unigenes with immunologic function were mapped into immune-related pathways. Expression of 41 unigenes measured by quantitative real-time PCR (qRT-PCR) showed consistent results with that of transcriptome analysis. When exposure challenge of Vibrio parahaemolyticus, it is indicated that the PI3K-AKT, MAPK, NF-κB and P53 signal pathways were involved in the hypoxia-induced immunosuppression of H. diversicolor. Furthermore, when the AKT gene (HdAKT) was inhibited by double-stranded RNA (dsRNA), expression levels of HdAKT was lower than the blank and control group in hemocytes at 4 h, 12 h and 24 h (p < 0.05).
中文翻译：
杂色鲍低氧应激下的免疫相关基因和信号通路：转录组分析。
由于气温和养殖密度的增加，热应激和缺氧应激已成为鲍鱼养殖面临的严重问题。压力会导致免疫抑制，这可能对水产养殖场造成严重的负面影响。为了研究缺氧应激和细菌攻击后免疫抑制的机制，对参与免疫的杂色菌血细胞的转录组进行了分析。总共生成了 307,395,572 个干净读取并组装成 99,774 个 unigene。 KEGG分析表明，225个具有免疫功能的unigenes被定位到免疫相关通路。通过实时定量PCR（qRT-PCR）测量的41个unigenes的表达与转录组分析的结果一致。副溶血性弧菌暴露攻击时，表明PI3K-AKT、MAPK、NF-κB和P53信号通路参与了杂色菌缺氧引起的免疫抑制。此外，当AKT基因（HdAKT）受到双链RNA（dsRNA）抑制时，4小时、12小时和24小时时血细胞中HdAKT的表达水平低于空白组和对照组（p％3C 0.05）。
更新日期：2019-12-25
中文翻译：
杂色鲍低氧应激下的免疫相关基因和信号通路：转录组分析。
由于气温和养殖密度的增加，热应激和缺氧应激已成为鲍鱼养殖面临的严重问题。压力会导致免疫抑制，这可能对水产养殖场造成严重的负面影响。为了研究缺氧应激和细菌攻击后免疫抑制的机制，对参与免疫的杂色菌血细胞的转录组进行了分析。总共生成了 307,395,572 个干净读取并组装成 99,774 个 unigene。 KEGG分析表明，225个具有免疫功能的unigenes被定位到免疫相关通路。通过实时定量PCR（qRT-PCR）测量的41个unigenes的表达与转录组分析的结果一致。副溶血性弧菌暴露攻击时，表明PI3K-AKT、MAPK、NF-κB和P53信号通路参与了杂色菌缺氧引起的免疫抑制。此外，当AKT基因（HdAKT）受到双链RNA（dsRNA）抑制时，4小时、12小时和24小时时血细胞中HdAKT的表达水平低于空白组和对照组（p％3C 0.05）。
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