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Zinc chloride influences embryonic development, growth, and Gh/Igf-1 gene expression during the early life stage in zebrafish (Danio rerio).
Comparative Biochemistry and Physiology C: Toxicology & Pharmacology ( IF 3.9 ) Pub Date : 2019-12-23 , DOI: 10.1016/j.cbpc.2019.108684 Yoshifumi Horie 1 , Kei Yonekura 1 , Ayaka Suzuki 1 , Chiho Takahashi 1
Comparative Biochemistry and Physiology C: Toxicology & Pharmacology ( IF 3.9 ) Pub Date : 2019-12-23 , DOI: 10.1016/j.cbpc.2019.108684 Yoshifumi Horie 1 , Kei Yonekura 1 , Ayaka Suzuki 1 , Chiho Takahashi 1
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Although zinc is an essential element for organisms, excess zinc exposure is harmful. We assessed the possible negative influence of zinc (Zn) on the freshwater fish Danio rerio during its early life stage by using Organization for Economic Cooperation and Development test guideline no. 210. Lethality of Zn after hatching occurred in a concentration dependent manner. The LC50 and lowest observed effect concentration of mortality values in the present toxicity assay were 2.31 mg/L (95% confidence limit: 1.81-3.05) and 1.5 mg/L, respectively. These values were close to the reported concentration recorded in aquatic environments. Growth inhibition was observed at 15 and 30 days post-hatching with Zn exposure of 1.5 mg/L. In general, the growth hormone (Gh)/insulin-like growth factor-I (Igf-1) axis is important for growth in fishes, and Zn exposure induced a significant reduction of igf-1 expression at the concentration that caused growth inhibition. These findings suggest that the observed growth inhibition was induced by the suppression of igf-1 expression. In addition, these results suggest that by examining gene expression on the Gh/Igf-1 axis, it may be possible to predict growth suppression by chemical exposure.
中文翻译：
氯化锌影响斑马鱼（Danio rerio）生命早期的胚胎发育，生长和Gh / Igf-1基因表达。
尽管锌是生物体必不可少的元素，但过量锌暴露是有害的。我们使用经济合作与发展组织（OECD）测试准则No. 1评估了锌（Zn）在淡水鱼Danio rerio生命早期阶段可能产生的负面影响。210.孵化后锌的致命性以浓度依赖的方式发生。在本毒性试验中，死亡值的LC50和最低观察到的效应浓度分别为2.31 mg / L（95％置信度：1.81-3.05）和1.5 mg / L。这些值接近在水生环境中记录的报告浓度。孵化后15天和30天观察到生长抑制，锌暴露为1.5 mg / L。一般来说，生长激素（Gh）/胰岛素样生长因子-I（Igf-1）轴对鱼类的生长很重要，锌暴露在引起生长抑制的浓度下导致igf-1表达显着降低。这些发现表明观察到的生长抑制是通过抑制igf-1表达诱导的。此外，这些结果表明，通过检查Gh / Igf-1轴上的基因表达，可能可以预测化学暴露对生长的抑制作用。
更新日期：2019-12-23
中文翻译：
氯化锌影响斑马鱼（Danio rerio）生命早期的胚胎发育，生长和Gh / Igf-1基因表达。
尽管锌是生物体必不可少的元素，但过量锌暴露是有害的。我们使用经济合作与发展组织（OECD）测试准则No. 1评估了锌（Zn）在淡水鱼Danio rerio生命早期阶段可能产生的负面影响。210.孵化后锌的致命性以浓度依赖的方式发生。在本毒性试验中，死亡值的LC50和最低观察到的效应浓度分别为2.31 mg / L（95％置信度：1.81-3.05）和1.5 mg / L。这些值接近在水生环境中记录的报告浓度。孵化后15天和30天观察到生长抑制，锌暴露为1.5 mg / L。一般来说，生长激素（Gh）/胰岛素样生长因子-I（Igf-1）轴对鱼类的生长很重要，锌暴露在引起生长抑制的浓度下导致igf-1表达显着降低。这些发现表明观察到的生长抑制是通过抑制igf-1表达诱导的。此外，这些结果表明，通过检查Gh / Igf-1轴上的基因表达，可能可以预测化学暴露对生长的抑制作用。
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