A Rhamnose-Inducible System for Precise and Temporal Control of Gene Expression in Cyanobacteria,ACS Synthetic Biology

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A Rhamnose-Inducible System for Precise and Temporal Control of Gene Expression in Cyanobacteria
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2018-03-15 00:00:00 , DOI: 10.1021/acssynbio.7b00435
Ciarán L. Kelly ₁ , George M. Taylor ₁ , Andrew Hitchcock ₁ , Antonio Torres-Méndez ₁ , John T. Heap ₁

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Cyanobacteria are important for fundamental studies of photosynthesis and have great biotechnological potential. In order to better study and fully exploit these organisms, the limited repertoire of genetic tools and parts must be expanded. A small number of inducible promoters have been used in cyanobacteria, allowing dynamic external control of gene expression through the addition of specific inducer molecules. However, the inducible promoters used to date suffer from various drawbacks including toxicity of inducers, leaky expression in the absence of inducer and inducer photolability, the latter being particularly relevant to cyanobacteria, which, as photoautotrophs, are grown under light. Here we introduce the rhamnose-inducible rhaBAD promoter of Escherichia coli into the model freshwater cyanobacterium Synechocystis sp. PCC 6803 and demonstrate it has superior properties to previously reported cyanobacterial inducible promoter systems, such as a non-toxic, photostable, non-metabolizable inducer, a linear response to inducer concentration and crucially no basal transcription in the absence of inducer.

中文翻译：

鼠李糖诱导系统的蓝藻中的基因表达的精确和时间控制。

蓝细菌对于光合作用的基础研究很重要，并且具有巨大的生物技术潜力。为了更好地研究和充分利用这些生物，必须扩大遗传工具和零件的有限种类。少数可诱导的启动子已用于蓝细菌中，通过添加特定的诱导剂分子，可以动态地外部控制基因的表达。然而，迄今为止使用的诱导型启动子具有多种缺点，包括诱导剂的毒性，在没有诱导剂的情况下的泄漏表达和诱导剂的可光化性，后者特别是与蓝细菌有关，蓝细菌作为光养生物，在光下生长。下面我们介绍鼠李糖诱导的rhaBAD启动子大肠杆菌进入模型淡水蓝藻Synechocystis sp.。PCC 6803并证明它具有比以前报道的蓝细菌可诱导启动子系统优越的性能，例如无毒，光稳定，不可代谢的诱导剂，对诱导剂浓度的线性响应以及在不存在诱导剂的情况下至关重要的基础转录。

更新日期：2018-03-15

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