Dextran Functionalization of Semiconducting Polymer Dots and Conjugation with Tetrameric Antibody Complexes for Bioanalysis and Imaging,ACS Applied Bio Materials

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Dextran Functionalization of Semiconducting Polymer Dots and Conjugation with Tetrameric Antibody Complexes for Bioanalysis and Imaging
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2019-12-20 , DOI: 10.1021/acsabm.9b00899
Kelsi Lix ₁ , Michael V Tran ₁ , Melissa Massey ₁ , Kelly Rees ₁ , Ethan R Sauvé ₁ , Zachary M Hudson ₁ , W Russ Algar ₁

Affiliation

Brightly fluorescent semiconducting polymer dots (Pdots) are emerging as very useful probes for bioanalysis and imaging. Unfortunately, Pdot materials often suffer from limitations such as poor colloidal and physical stability, nonspecific adsorption, and relatively few reported surface chemistries and bioconjugate chemistries. To help address these limitations, we have developed dextran-functionalized Pdots (Dex-Pdots). This functionalization improves particle stability over a range of pH and at high ionic strength, hinders surface-induced unfolding, and enables the preparation of immunoconjugates via tetrameric antibody complexes (TAC). The utility of TAC-conjugated Dex-Pdots is demonstrated through a proof-of-concept fluorescence-linked immunosorbent assay (FLISA) for human erythropoietin (EPO), and through immunolabeling of human epidermal growth factor receptor 2 (HER2)-positive SK-BR3 breast cancer cells. The conjugates exhibited less nonspecific binding and greater specific binding than Pdots without dextran functionalization. Overall, dextran functionalization is a highly promising surface chemistry for biological applications of Pdots.

中文翻译：

用于生物分析和成像的半导体聚合物点的葡聚糖功能化和与四聚体抗体复合物的缀合

明亮的荧光半导体聚合物点 (Pdots) 正在成为非常有用的生物分析和成像探针。不幸的是，Pdot 材料经常受到限制，例如胶体和物理稳定性差、非特异性吸附，以及报道的表面化学和生物共轭化学相对较少。为了帮助解决这些限制，我们开发了葡聚糖功能化 Pdots (Dex-Pdots)。这种功能化提高了粒子在一定 pH 范围内和高离子强度下的稳定性，阻碍了表面诱导的展开，并能够通过四聚体抗体复合物 (TAC) 制备免疫偶联物。TAC 缀合的 Dex-Pdots 的效用通过人类促红细胞生成素 (EPO) 的概念验证荧光关联免疫吸附测定 (FLISA) 得到证明，并通过免疫标记人表皮生长因子受体 2 (HER2) 阳性 SK-BR3 乳腺癌细胞。与没有葡聚糖官能化的 Pdot 相比，缀合物表现出更少的非特异性结合和更高的特异性结合。总体而言，葡聚糖功能化是一种非常有前途的表面化学，可用于 Pdot 的生物学应用。

更新日期：2019-12-21

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