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Jatrorrhizine Balances the Gut Microbiota and Reverses Learning and Memory Deficits in APP/PS1 transgenic mice.
Scientific Reports ( IF 3.8 ) Pub Date : 2019-12-20 , DOI: 10.1038/s41598-019-56149-9 Sheng Wang 1 , Wei Jiang 2 , Ting Ouyang 1 , Xiu-Yin Shen 1 , Fen Wang 1 , Yu-Hua Qu 3 , Min Zhang 1 , Tao Luo 1 , Hua-Qiao Wang 1
Scientific Reports ( IF 3.8 ) Pub Date : 2019-12-20 , DOI: 10.1038/s41598-019-56149-9 Sheng Wang 1 , Wei Jiang 2 , Ting Ouyang 1 , Xiu-Yin Shen 1 , Fen Wang 1 , Yu-Hua Qu 3 , Min Zhang 1 , Tao Luo 1 , Hua-Qiao Wang 1
Affiliation
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Alzheimer's disease (AD) is a complex disorder influenced by both genetic and environmental components and has become a major public health issue throughout the world. Oxidative stress and inflammation play important roles in the evolution of those major pathological symptoms. Jatrorrhizine (JAT), a main component of a traditional Chinese herbal, coptidis rhizome, has been shown to have neuroprotective effects and we previously showed that it is also able to clear oxygen free radicals and reduce inflammatory responses. In this study, we demonstrated that JAT administration could alleviate the learning and memory deficits in AD. Furthermore, we also found that JAT treatment reduced the levels of Aβ plaques in the cortex and hippocampus of APP/PS1 double-transgenic mice. Other studies suggest that there are gut microbiome alterations in AD. In order to explore the underlying mechanisms between gut microbiota and AD, DNA sequencing for 16s rDNA V3-V4 was performed in fecal samples from APP/PS1 transgenic mice and C57BL/6 wild-type (WT) mice. Our results indicated that APP/PS1 mice showed less Operational Taxonomic Units (OTUs) abundance in gut microbiota than WT mice and with different composition. Furthermore, JAT treatment enriched OTUs abundance and alpha diversity in APP/PS1 mice compared to WT mice. High dose of JAT treatment altered the abundance of some specific gut microbiota such as the most predominant phylum Firmicutes and Bacteroidetes in APP/PS1 mice. In conclusion, APP/PS1 mice display gut dysbiosis, and JAT treatment not only improved the memory deficits, but also regulated the abundance of the microbiota. This may provide a therapeutic way to balance the gut dysbiosis in AD patients.
中文翻译：
麻风树碱平衡APP / PS1转基因小鼠的肠道菌群并逆转学习和记忆障碍。
阿尔茨海默氏病（AD）是一种受遗传和环境因素影响的复杂疾病，已成为世界范围内的主要公共卫生问题。氧化应激和炎症在那些主要病理症状的演变中起重要作用。麻风树碱（JAT）是传统中草药黄连的主要成分，已被证明具有神经保护作用，而我们之前的研究表明，它还能够清除氧自由基并减少炎症反应。在这项研究中，我们证明了JAT管理可以缓解AD的学习和记忆障碍。此外，我们还发现JAT处理可降低APP / PS1双转基因小鼠皮质和海马中Aβ斑块的水平。其他研究表明，AD中存在肠道微生物组改变。为了探索肠道菌群和AD之间的潜在机制，在APP / PS1转基因小鼠和C57BL / 6野生型（WT）小鼠的粪便样品中进行了16s rDNA V3-V4的DNA测序。我们的结果表明，APP / PS1小鼠在肠道菌群中显示出比WT小鼠更少的操作分类单位（OTUs）丰度，并且具有不同的组成。此外，与WT小鼠相比，JAT处理在APP / PS1小鼠中丰富了OTU的丰度和alpha多样性。高剂量的JAT处理可改变APP / PS1小鼠中某些特定肠道菌群的丰度，例如最主要的门菌纲和拟杆菌。总之，APP / PS1小鼠表现出肠道营养不良，而JAT处理不仅可以改善记忆缺陷，还可以调节微生物群的丰度。
更新日期：2019-12-20
中文翻译：
麻风树碱平衡APP / PS1转基因小鼠的肠道菌群并逆转学习和记忆障碍。
阿尔茨海默氏病（AD）是一种受遗传和环境因素影响的复杂疾病，已成为世界范围内的主要公共卫生问题。氧化应激和炎症在那些主要病理症状的演变中起重要作用。麻风树碱（JAT）是传统中草药黄连的主要成分，已被证明具有神经保护作用，而我们之前的研究表明，它还能够清除氧自由基并减少炎症反应。在这项研究中，我们证明了JAT管理可以缓解AD的学习和记忆障碍。此外，我们还发现JAT处理可降低APP / PS1双转基因小鼠皮质和海马中Aβ斑块的水平。其他研究表明，AD中存在肠道微生物组改变。为了探索肠道菌群和AD之间的潜在机制，在APP / PS1转基因小鼠和C57BL / 6野生型（WT）小鼠的粪便样品中进行了16s rDNA V3-V4的DNA测序。我们的结果表明，APP / PS1小鼠在肠道菌群中显示出比WT小鼠更少的操作分类单位（OTUs）丰度，并且具有不同的组成。此外，与WT小鼠相比，JAT处理在APP / PS1小鼠中丰富了OTU的丰度和alpha多样性。高剂量的JAT处理可改变APP / PS1小鼠中某些特定肠道菌群的丰度，例如最主要的门菌纲和拟杆菌。总之，APP / PS1小鼠表现出肠道营养不良，而JAT处理不仅可以改善记忆缺陷，还可以调节微生物群的丰度。
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