A facile analytical method for reliable selectivity examination in cofactor NADH regeneration.,Chemical Communications

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A facile analytical method for reliable selectivity examination in cofactor NADH regeneration.
Chemical Communications ( IF 4.3 ) Pub Date : 2020-01-03 , DOI: 10.1039/c9cc07805c
Tony Saba ₁ , Joseph W H Burnett , Jianwei Li , Panagiotis N Kechagiopoulos , Xiaodong Wang

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This study demonstrates a novel method to quantify selective (1,4-NADH) and unselective products (1,2- and 1,6-NADH) in NADH regeneration using combined UV-Vis spectroscopy and biological assays. The validity of the proposed method was tested in the Pt/C promoted NAD+ hydrogenation using hydrogen as reducing agent.

中文翻译：

一种在辅助因子NADH再生中进行可靠选择性检测的简便分析方法。

这项研究演示了一种新方法，可通过结合使用紫外-可见光谱和生物学分析来定量NADH再生中的选择性（1,4-NADH）和非选择性产物（1,2-和1,6-NADH）。在以氢为还原剂的Pt / C促进的NAD +加氢中测试了该方法的有效性。

更新日期：2020-01-04

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