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Porous rice starch produced by combined ultrasound-assisted ice recrystallization and enzymatic hydrolysis.
International Journal of Biological Macromolecules ( IF 7.7 ) Pub Date : 2019-12-18 , DOI: 10.1016/j.ijbiomac.2019.12.144 Thewika Keeratiburana 1 , Aleksander Riise Hansen 2 , Siriwat Soontaranon 3 , Sunanta Tongta 4 , Andreas Blennow 2
International Journal of Biological Macromolecules ( IF 7.7 ) Pub Date : 2019-12-18 , DOI: 10.1016/j.ijbiomac.2019.12.144 Thewika Keeratiburana 1 , Aleksander Riise Hansen 2 , Siriwat Soontaranon 3 , Sunanta Tongta 4 , Andreas Blennow 2
Affiliation
The effects of multicycle ultrasound-assisted ice recrystallization (US+IR) combined with amyloglucosidase (AMG) or maltogenic α-amylase (MA) catalyzed hydrolysis on structure were investigated. Scanning electron microscopy (SEM) showed that the US+IR produced shallow indentations and grooves on the exterior of granules while the combination US+IR and enzyme hydrolysis created additional pores on starch granules. MA displayed a higher number of pores than AMG. The highest values of specific surface area (SBET) and the total pore volume were obtained for US+IR→MA (1.96 m2 g-1 and 7.26 × 10-3 cm3 g-1, respectively). The US+IR treatment significantly decreased the relative crystallinity, amylose content and swelling capacity. Those parameters were further efficiently decreased following enzymatic hydrolysis. The combined treatments generated products with higher initial gelatinization temperature (Ti) compared to the corresponding controls. The US+IR increased the digestion rate constant (k-value) compared to native starch. However, the combined treatment, US+IR→AMG, significantly decreased the k-value from 2.97 × 10-3 to 2.50 × 10-3 min-1 compared to its control. Our study demonstrates that US+IR treatment in combination with enzyme hydrolysis is a useful method to produce specifically functionalized porous rice starch that can be used as e.g. absorbents and for further chemical modifications.
中文翻译:
超声波辅助冰重结晶和酶水解相结合产生的多孔米淀粉。
研究了多周期超声辅助冰重结晶(US + IR)结合淀粉葡糖苷酶(AMG)或麦芽糖α-淀粉酶(MA)催化水解对结构的影响。扫描电子显微镜(SEM)显示,US + IR在颗粒的外部产生浅凹痕和凹槽,而US + IR和酶水解的组合在淀粉颗粒上产生了额外的孔。MA的毛孔数量比AMG多。对于US + IR→MA,获得了最高的比表面积(SBET)和总孔体积值(分别为1.96 m2 g-1和7.26×10-3 cm3 g-1)。US + IR处理显着降低了相对结晶度,直链淀粉含量和溶胀能力。在酶促水解之后,那些参数被进一步有效地降低。与相应的对照相比,联合处理产生的产品具有更高的初始糊化温度(Ti)。与天然淀粉相比,US + IR增加了消化速率常数(k值)。但是,与对照组相比,US + IR→AMG的联合处理将k值从2.97×10-3显着降低到2.50×10-3 min-1。我们的研究表明,US + IR处理与酶水解相结合是生产特别功能化的多孔大米淀粉的一种有用方法,该淀粉可以用作例如吸收剂并用于进一步的化学修饰。与对照组相比,k值从2.97×10-3显着降低到2.50×10-3 min-1。我们的研究表明,US + IR处理与酶水解相结合是生产特别功能化的多孔大米淀粉的一种有用方法,该淀粉可以用作例如吸收剂并用于进一步的化学修饰。与对照组相比,k值从2.97×10-3显着降低到2.50×10-3 min-1。我们的研究表明,US + IR处理与酶水解相结合是生产特别功能化的多孔大米淀粉的一种有用方法,该淀粉可以用作例如吸收剂并用于进一步的化学修饰。
更新日期:2019-12-18
中文翻译:
超声波辅助冰重结晶和酶水解相结合产生的多孔米淀粉。
研究了多周期超声辅助冰重结晶(US + IR)结合淀粉葡糖苷酶(AMG)或麦芽糖α-淀粉酶(MA)催化水解对结构的影响。扫描电子显微镜(SEM)显示,US + IR在颗粒的外部产生浅凹痕和凹槽,而US + IR和酶水解的组合在淀粉颗粒上产生了额外的孔。MA的毛孔数量比AMG多。对于US + IR→MA,获得了最高的比表面积(SBET)和总孔体积值(分别为1.96 m2 g-1和7.26×10-3 cm3 g-1)。US + IR处理显着降低了相对结晶度,直链淀粉含量和溶胀能力。在酶促水解之后,那些参数被进一步有效地降低。与相应的对照相比,联合处理产生的产品具有更高的初始糊化温度(Ti)。与天然淀粉相比,US + IR增加了消化速率常数(k值)。但是,与对照组相比,US + IR→AMG的联合处理将k值从2.97×10-3显着降低到2.50×10-3 min-1。我们的研究表明,US + IR处理与酶水解相结合是生产特别功能化的多孔大米淀粉的一种有用方法,该淀粉可以用作例如吸收剂并用于进一步的化学修饰。与对照组相比,k值从2.97×10-3显着降低到2.50×10-3 min-1。我们的研究表明,US + IR处理与酶水解相结合是生产特别功能化的多孔大米淀粉的一种有用方法,该淀粉可以用作例如吸收剂并用于进一步的化学修饰。与对照组相比,k值从2.97×10-3显着降低到2.50×10-3 min-1。我们的研究表明,US + IR处理与酶水解相结合是生产特别功能化的多孔大米淀粉的一种有用方法,该淀粉可以用作例如吸收剂并用于进一步的化学修饰。