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Insight into the biology of Mycobacterium mucogenicum and Mycobacterium neoaurum clade members.
Scientific Reports ( IF 3.8 ) Pub Date : 2019-12-17 , DOI: 10.1038/s41598-019-55464-5 Phani Rama Krishna Behra 1 , B M Fredrik Pettersson 1 , Malavika Ramesh 1 , Santanu Dasgupta 1 , Leif A Kirsebom 1
Scientific Reports ( IF 3.8 ) Pub Date : 2019-12-17 , DOI: 10.1038/s41598-019-55464-5 Phani Rama Krishna Behra 1 , B M Fredrik Pettersson 1 , Malavika Ramesh 1 , Santanu Dasgupta 1 , Leif A Kirsebom 1
Affiliation
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Nontuberculous mycobacteria, NTM, are of growing concern and among these members of the Mycobacterium mucogenicum (Mmuc) and Mycobacterium neoaurum (Mneo) clades can cause infections in humans and they are resistant to first-line anti-tuberculosis drugs. They can be isolated from different ecological niches such as soil, tap water and ground water. Mycobacteria, such as Mmuc and Mneo, are classified as rapid growing mycobacteria, RGM, while the most familiar, Mycobacterium tuberculosis, belongs to the slow growing mycobacteria, SGM. Modern "omics" approaches have provided new insights into our understanding of the biology and evolution of this group of bacteria. Here we present comparative genomics data for seventeen NTM of which sixteen belong to the Mmuc- and Mneo-clades. Focusing on virulence genes, including genes encoding sigma/anti-sigma factors, serine threonine protein kinases (STPK), type VII (ESX genes) secretion systems and mammalian cell entry (Mce) factors we provide insight into their presence as well as phylogenetic relationship in the case of the sigma/anti-sigma factors and STPKs. Our data further suggest that these NTM lack ESX-5 and Mce2 genes, which are known to affect virulence. In this context, Mmuc- and Mneo-clade members lack several of the genes in the glycopeptidolipid (GLP) locus, which have roles in colony morphotype appearance and virulence. For the M. mucogenicum type strain, MmucT, we provide RNASeq data focusing on mRNA levels for sigma factors, STPK, ESX proteins and Mce proteins. These data are discussed and compared to in particular the SGM and fish pathogen Mycobacterium marinum. Finally, we provide insight into as to why members of the Mmuc- and Mneo-clades show resistance to rifampin and isoniazid, and why MmucT forms a rough colony morphotype.
中文翻译：
深入了解粘液分枝杆菌和新分枝杆菌分支成员的生物学。
非结核分枝杆菌（NTM）引起了越来越多的关注，在粘液分枝杆菌（Mmuc）和新分枝杆菌（Mneo）进化枝的这些成员中，它们可引起人类感染，并且它们对一线抗结核药物具有耐药性。它们可以与不同的生态位隔离，例如土壤，自来水和地下水。分枝杆菌，例如Mmuc和Mneo，被归类为快速生长的分枝杆菌，RGM，而最常见的结核分枝杆菌属于慢速生长的分枝杆菌，SGM。现代“组学”方法为我们对这组细菌的生物学和进化的理解提供了新的见识。在这里，我们介绍了17个NTM的比较基因组学数据，其中16个属于Mmuc-和Mneo-clade。专注于毒性基因，包括编码sigma / anti-sigma因子的基因，丝氨酸苏氨酸蛋白激酶（STPK），VII型（ESX基因）分泌系统和哺乳动物细胞进入（Mce）因子，我们提供了对它们的存在以及系统发育关系的见解。 sigma / anti-sigma因子和STPK。我们的数据进一步表明，这些NTM缺乏已知会影响毒力的ESX-5和Mce2基因。在这种情况下，Mmuc-和Mneo-clade成员缺少糖肽脂质（GLP）基因座中的几个基因，这些基因在菌落形态型和毒力中起作用。对于粘多糖分枝杆菌型菌株MmucT，我们提供RNASeq数据，重点关注sigma因子，STPK，ESX蛋白和Mce蛋白的mRNA水平。讨论并比较了这些数据，特别是与SGM和鱼类病原体海洋分枝杆菌。最后，
更新日期：2019-12-18
中文翻译：
深入了解粘液分枝杆菌和新分枝杆菌分支成员的生物学。
非结核分枝杆菌（NTM）引起了越来越多的关注，在粘液分枝杆菌（Mmuc）和新分枝杆菌（Mneo）进化枝的这些成员中，它们可引起人类感染，并且它们对一线抗结核药物具有耐药性。它们可以与不同的生态位隔离，例如土壤，自来水和地下水。分枝杆菌，例如Mmuc和Mneo，被归类为快速生长的分枝杆菌，RGM，而最常见的结核分枝杆菌属于慢速生长的分枝杆菌，SGM。现代“组学”方法为我们对这组细菌的生物学和进化的理解提供了新的见识。在这里，我们介绍了17个NTM的比较基因组学数据，其中16个属于Mmuc-和Mneo-clade。专注于毒性基因，包括编码sigma / anti-sigma因子的基因，丝氨酸苏氨酸蛋白激酶（STPK），VII型（ESX基因）分泌系统和哺乳动物细胞进入（Mce）因子，我们提供了对它们的存在以及系统发育关系的见解。 sigma / anti-sigma因子和STPK。我们的数据进一步表明，这些NTM缺乏已知会影响毒力的ESX-5和Mce2基因。在这种情况下，Mmuc-和Mneo-clade成员缺少糖肽脂质（GLP）基因座中的几个基因，这些基因在菌落形态型和毒力中起作用。对于粘多糖分枝杆菌型菌株MmucT，我们提供RNASeq数据，重点关注sigma因子，STPK，ESX蛋白和Mce蛋白的mRNA水平。讨论并比较了这些数据，特别是与SGM和鱼类病原体海洋分枝杆菌。最后，
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