Aggregation-induced fluorescence of the luminol-terbium(III) complex in polymer nanoparticles for sensitive determination of thrombin,Microchimica Acta

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Aggregation-induced fluorescence of the luminol-terbium(III) complex in polymer nanoparticles for sensitive determination of thrombin
Microchimica Acta ( IF 5.3 ) Pub Date : 2019-12-17 , DOI: 10.1007/s00604-019-4043-z
Yuan-Jun Tong ₁ , An-Min Song ₁ , Lu-Dan Yu ₁ , Ru-Ping Liang ₁ , Jian-Ding Qiu _{1,

2}

Affiliation

A fluorometric method is described for the determination of thrombin. Polymer nanoparticles containing the luminol-terbium(III) complex (luminol-Tb) were prepared where luminol acts as the bridging ligand, and Tb(III) acts as the central metal ion. Thrombin possesses a large number of electrons donating groups that coordinate with luminol-Tb. Following coordination, the rigidity of the linker is increased, and this decreases the non-radiative decay rate and induces an increase in fluorescence intensity at 430 nm. Hence, thrombin can be fluorometrically determined. The detection limit of thrombin is as low as 3.5 pM (at an SNR of 3). This is about 10 times lower than assays using an aptamer. The method was applied in the determination of thrombin in human serum via the standard addition method and gave satisfying results. Graphical abstract Schematic representation of the preparation of the luminol-Tb(III) complex in a nanoparticle host by the self-assembly of luminol and Tb(III) ions. Thrombin readily coordinates with the luminol-Tb(III) system, and this results in particle aggregation. The blue fluorescence of luminol increases strongly, and this effect provides the basis for fluorometric determination of thrombin. Schematic representation of the preparation of the luminol-Tb(III) complex in a nanoparticle host by the self-assembly of luminol and Tb(III) ions. Thrombin readily coordinates with the luminol-Tb(III) system, and this results in particle aggregation. The blue fluorescence of luminol increases strongly, and this effect provides the basis for fluorometric determination of thrombin.

中文翻译：

聚合物纳米粒子中鲁米诺-铽 (III) 复合物的聚集诱导荧光用于灵敏测定凝血酶

描述了一种用于测定凝血酶的荧光法。制备了含有鲁米诺-铽 (III) 复合物 (鲁米诺-Tb) 的聚合物纳米粒子，其中鲁米诺作为桥接配体，Tb(III) 作为中心金属离子。凝血酶拥有大量与鲁米诺-Tb 协调的供电子基团。协调后，接头的刚性增加，这降低了非辐射衰减率，并导致 430 nm 处的荧光强度增加。因此，可以荧光测定法测定凝血酶。凝血酶的检测限低至 3.5 pM（SNR 为 3）。这比使用适体的测定低约 10 倍。该方法用于标准加入法测定人血清中凝血酶，结果令人满意。图形摘要通过鲁米诺和 Tb(III) 离子的自组装在纳米颗粒主体中制备鲁米诺-Tb(III) 复合物的示意图。凝血酶很容易与鲁米诺-Tb(III) 系统协调，这会导致颗粒聚集。鲁米诺的蓝色荧光强烈增强，这种效应为凝血酶的荧光测定提供了依据。通过鲁米诺和 Tb(III) 离子的自组装在纳米粒子宿主中制备鲁米诺-Tb(III) 复合物的示意图。凝血酶很容易与鲁米诺-Tb(III) 系统协调，这会导致颗粒聚集。鲁米诺的蓝色荧光强烈增强，这种效应为凝血酶的荧光测定提供了依据。

更新日期：2019-12-17

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