The Arabidopsis Transcriptome Responds Specifically and Dynamically to High Light Stress.,Cell Reports

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The Arabidopsis Transcriptome Responds Specifically and Dynamically to High Light Stress.
Cell Reports ( IF 7.5 ) Pub Date : 2019-12-17 , DOI: 10.1016/j.celrep.2019.11.051
Jianyan Huang ₁ , Xiaobo Zhao ₂ , Joanne Chory ₁

Affiliation

The dynamic and specific transcriptome for high light (HL) stress in plants is poorly understood because heat has confounded previous studies. Here, we perform an in-depth temporal responsive transcriptome analysis and identify the core HL-responsive genes. By eliminating the effect of heat, we uncover a set of genes specifically regulated by high-intensity light-driven signaling. We find that 79% of HL-responsive genes restore their expression to baseline within a 14-h recovery period. Our study reveals that plants respond to HL through dynamic regulation of hormones, particularly abscisic acid (ABA), photosynthesis, and phenylpropanoid pathway genes. Blue/UV-A photoreceptors and phytochrome-interacting factor (PIF) genes are also responsive to HL. We further show that ABA biosynthesis-defective mutant nced3nced5, as well as pif4, pif5, pif4,5, and pif1,3,4,5 mutants, are hypersensitive to HL. Our study presents the dynamic and specific high-intensity light-driven transcriptional landscape in plants during HL stress.

中文翻译：

拟南芥转录组对高光胁迫有特异性和动态反应。

对植物中高光 (HL) 胁迫的动态和特异性转录组知之甚少，因为热量混淆了以前的研究。在这里，我们进行了深入的时间响应转录组分析并确定了核心 HL 响应基因。通过消除热的影响，我们发现了一组受高强度光驱动信号特别调控的基因。我们发现 79% 的 HL 反应基因在 14 小时的恢复期内将其表达恢复到基线。我们的研究表明，植物通过动态调节激素，特别是脱落酸 (ABA)、光合作用和苯丙烷途径基因来响应 HL。蓝色/UV-A 光感受器和光敏色素相互作用因子 (PIF) 基因也对 HL 有反应。我们进一步表明 ABA 生物合成缺陷突变体 nced3nced5 以及 pif4、pif5、pif4,5、和 pif1,3,4,5 突变体对 HL 过敏。我们的研究展示了 HL 胁迫期间植物中动态和特定的高强度光驱动转录景观。

更新日期：2019-12-18

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