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The Catenulida flatworm can express genes from its microbiome or from the DNA it ingests.
Scientific Reports ( IF 3.8 ) Pub Date : 2019-12-13 , DOI: 10.1038/s41598-019-55659-w Marcos Trindade Rosa 1 , Elgion L S Loreto 2
Scientific Reports ( IF 3.8 ) Pub Date : 2019-12-13 , DOI: 10.1038/s41598-019-55659-w Marcos Trindade Rosa 1 , Elgion L S Loreto 2
Affiliation
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Stenostomum are tiny planarians of the phylum Platyhelminthes that reproduce asexually. We transfected these worms using plasmids containing a gfp reporter gene. Here we show that they can express genes present in plasmids carried by bacteria and those that are encoded by naked DNA, such as plasmids or PCR fragments, transfected by electroporation; they can also express genes taken up during feeding. The microbiome associated with worm maintenance was evaluated, and the results indicated that when a plasmid is maintained in the microbiome, gfp gene expression is stable. When genes originate from naked DNA or bacteria not maintained in the microbiome, GFP expression is transient. Therefore, changes in the microbiome can modify the ability of worms to express foreign genes. In stable GFP-expressing worms, NSG showed that the gfp gene was maintained in the plasmid and was not integrated into the chromosome. These results suggest that, at least for some organisms such as flatworms, the expression of genes provided by the microbiome or the environment can be considered among the potential sources of phenotypic plasticity, which can have implications for evolvability.
中文翻译：
Catenulida扁虫可以从其微生物组或从其摄入的DNA中表达基因。
狭窄的口齿是侧柏的细小平面虫，无性繁殖。我们使用包含gfp报告基因的质粒转染了这些蠕虫。在这里，我们表明它们可以表达细菌携带的质粒中存在的基因，以及通过电穿孔转染的裸DNA编码的基因，例如质粒或PCR片段；它们还可以表达进食过程中摄取的基因。评价了与蠕虫维持相关的微生物组，结果表明当质粒保留在微生物组中时，gfp基因表达是稳定的。当基因来自裸露的DNA或微生物组中不存在的细菌时，GFP的表达是瞬时的。因此，微生物组的变化可以改变蠕虫表达外源基因的能力。在稳定的GFP表达蠕虫中，NSG显示gfp基因保留在质粒中，没有整合到染色体中。这些结果表明，至少对于某些生物，例如扁虫，可以考虑由微生物组或环境提供的基因表达，这可能是表型可塑性的潜在来源，这可能影响其可进化性。
更新日期：2019-12-13
中文翻译：
Catenulida扁虫可以从其微生物组或从其摄入的DNA中表达基因。
狭窄的口齿是侧柏的细小平面虫，无性繁殖。我们使用包含gfp报告基因的质粒转染了这些蠕虫。在这里，我们表明它们可以表达细菌携带的质粒中存在的基因，以及通过电穿孔转染的裸DNA编码的基因，例如质粒或PCR片段；它们还可以表达进食过程中摄取的基因。评价了与蠕虫维持相关的微生物组，结果表明当质粒保留在微生物组中时，gfp基因表达是稳定的。当基因来自裸露的DNA或微生物组中不存在的细菌时，GFP的表达是瞬时的。因此，微生物组的变化可以改变蠕虫表达外源基因的能力。在稳定的GFP表达蠕虫中，NSG显示gfp基因保留在质粒中，没有整合到染色体中。这些结果表明，至少对于某些生物，例如扁虫，可以考虑由微生物组或环境提供的基因表达，这可能是表型可塑性的潜在来源，这可能影响其可进化性。
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