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A microemulsion high-performance liquid chromatography (MELC) method for the separation and determination of hydrolyzed tenuifolin in Radix Polygalae.
Scientific Reports ( IF 3.8 ) Pub Date : 2019-12-13 , DOI: 10.1038/s41598-019-55416-z Hui Yan 1 , Zhuan-Di Zheng 1 , Hong-Fei Wu 1 , Xiao-Chuang Liu 2 , An Zhou 1, 3
Scientific Reports ( IF 3.8 ) Pub Date : 2019-12-13 , DOI: 10.1038/s41598-019-55416-z Hui Yan 1 , Zhuan-Di Zheng 1 , Hong-Fei Wu 1 , Xiao-Chuang Liu 2 , An Zhou 1, 3
Affiliation
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Tenuifolin was used as a reliable chemical marker for the quality control of Radix Polygalae. The determination of tenuifolin is challenging because the analyte molecule lacks a suitable chromophore. The aim of this study was to establish a microemulsion high-performance liquid chromatography (MELC) method which is robust and sensitive, and can separate and determine tenuifolin in Radix Polygalae using an oil-in-water (O/W) microemulsion mobile phase. The separations were performed on a C18 (4.6 × 250 mm, 5 μm) column at 25 °C using a flow rate of 1.0 mL/min, and an ultraviolet detection wavelength of 210 nm. The microemulsion mobile phase comprised 2.8% (w/v) sodium dodecyl sulfate (SDS), 7.0% (v/v) n-butanol, 0.8% (v/v) n-octane and 0.1% (v/v) aqueous orthophosphate buffer (H3PO4). The linearity analysis of tenuifolin showed a correlation coefficient of 0.9923 in the concentration range of 48.00-960.00 µg/mL. The accuracy of the method based on three concentration levels ranged from 96.23% to 99.28%; the limit of detection (LOD) was 2.34 µg/mL, and the limit of quantification (LOQ) was 6.76 µg/mL. The results of our study indicated that the optimized MELC method was sensitive and robust, and can be widely applied for the separation and determination of tenuifolin in Radix Polygalae.
中文翻译：
微乳高效液相色谱(MELC)方法分离测定远志中水解细叶草苷的含量。
Tenuifolin 被用作远志质量控制的可靠化学标记物。由于分析物分子缺乏合适的发色团，特纽叶林的测定具有挑战性。本研究的目的是建立一种稳健、灵敏的微乳高效液相色谱（MELC）方法，使用水包油（O/W）微乳流动相分离和测定远志中的千叶草苷。分离采用 C18（4.6 × 250 mm，5 μm）柱，温度 25 °C，流速 1.0 mL/min，紫外检测波长 210 nm。微乳液流动相包含 2.8% (w/v) 十二烷基硫酸钠 (SDS)、7.0% (v/v) 正丁醇、0.8% (v/v) 正辛烷和 0.1% (v/v) 正磷酸盐水溶液缓冲液（H3PO4）。细叶草苷的线性分析显示，在48.00-960.00 µg/mL的浓度范围内，相关系数为0.9923。基于三个浓度水平的方法准确度为96.23%~99.28%；检测限 (LOD) 为 2.34 µg/mL，定量限 (LOQ) 为 6.76 µg/mL。研究结果表明，优化后的MELC方法灵敏、稳健，可广泛应用于远志中青叶苷的分离测定。
更新日期：2019-12-13
中文翻译：
微乳高效液相色谱(MELC)方法分离测定远志中水解细叶草苷的含量。
Tenuifolin 被用作远志质量控制的可靠化学标记物。由于分析物分子缺乏合适的发色团，特纽叶林的测定具有挑战性。本研究的目的是建立一种稳健、灵敏的微乳高效液相色谱（MELC）方法，使用水包油（O/W）微乳流动相分离和测定远志中的千叶草苷。分离采用 C18（4.6 × 250 mm，5 μm）柱，温度 25 °C，流速 1.0 mL/min，紫外检测波长 210 nm。微乳液流动相包含 2.8% (w/v) 十二烷基硫酸钠 (SDS)、7.0% (v/v) 正丁醇、0.8% (v/v) 正辛烷和 0.1% (v/v) 正磷酸盐水溶液缓冲液（H3PO4）。细叶草苷的线性分析显示，在48.00-960.00 µg/mL的浓度范围内，相关系数为0.9923。基于三个浓度水平的方法准确度为96.23%~99.28%；检测限 (LOD) 为 2.34 µg/mL，定量限 (LOQ) 为 6.76 µg/mL。研究结果表明，优化后的MELC方法灵敏、稳健，可广泛应用于远志中青叶苷的分离测定。
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