酶诱导分子印迹聚合物在 SERS 底物上用于超灵敏检测展青霉素,Analytica Chimica Acta

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酶诱导分子印迹聚合物在 SERS 底物上用于超灵敏检测展青霉素
Analytica Chimica Acta ( IF 5.7 ) Pub Date : 2020-03-01 , DOI: 10.1016/j.aca.2019.12.030
Yuanyuan Zhu ₁ , Long Wu ₁ , Heng Yan ₂ , Zhicheng Lu ₁ , Wenmin Yin ₁ , Heyou Han ₁

Affiliation

我们通过结合分子印迹聚合物 (MIP) 选择性和 SERS 技术灵敏度，设计了一种新型 MIP-SERS 底物，用于对展青霉素 (PAT) 进行特异性和无标记检测。最初，PDMS/AAO 的固体基材是使用聚二甲基硅氧烷 (PDMS) 混凝土阳极氧化铝 (AAO) 模板制备的。然后在 PDMS/AAO 表面溅射适量的 Au 以获得 Au/PDMS/AAO SERS 衬底。基于HRP酶在Au/PDMS/AAO上引发的原位聚合，成功合成了具有选择性聚合物和高张力SERS“热点”的MIP-SERS底物。新的 MIP-SERS 基底表现出很强的 SERS 增强效果和良好的 PAT 选择性。此外，结果表明该方法的线性范围为 5 × 10-10 至 10-6 M，检出限 (LOD) 为 8。PAT 为 5 × 10-11 M (S/N = 3)。所提出的方法还表现出可接受的重现性（相对标准偏差，RSD = 4.7%），良好的稳定性（两周后拉曼强度在 80% 以上），回收率从 96.43% 到 112.83%，平均 RSD 为 6.3%。该底物易于使用，无需复杂的样品预处理，这使其成为食品样品中快速灵敏检测方法的潜在候选者。

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Enzyme induced molecularly imprinted polymer on SERS substrate for ultrasensitive detection of patulin

We designed a new type of MIP-SERS substrate for specific and label-free detection of patulin (PAT), by combining molecular imprinting polymer (MIP) selectivity and SERS technology sensitivity. Initially, the solid substrate of PDMS/AAO was prepared using poly dimethylsiloxane (PDMS) concreted anodized aluminum oxide (AAO) template. Then moderate Au was sputtered on the surface of PDMS/AAO to obtain Au/PDMS/AAO SERS substrate. Based on the HRP enzyme initiated in situ polymerization on the Au/PDMS/AAO, the MIP-SERS substrate was successfully synthesized with selective polymer and high tense of SERS "hot spots". The new MIP-SERS substrate showed strong SERS enhancement effect and good selectivity for PAT. Besides, the results showed that the method owned a linear range from 5 × 10-10 to 10-6 M with the limit of detection (LOD) of 8.5 × 10-11 M (S/N = 3) for PAT. The proposed method also exhibited acceptable reproducibility (relative standard deviation, RSD = 4.7%)，good stability (Raman intensity is above 80% after two weeks) and recoveries from 96.43% to 112.83% with the average RSD of 6.3%. The substrate is easy to use without complex sample pretreatment, which makes it a potential candidate as a rapid and sensitive detection method in food samples.

更新日期：2020-03-01

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