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Flavin-mediated extracellular electron transfer in Gram-positive bacteria Bacillus cereus DIF1 and Rhodococcus ruber DIF2
RSC Advances ( IF 3.9 ) Pub Date : 2019-12-11 , DOI: 10.1039/c9ra08045g Tian Tian 1 , Xiaoyang Fan 1 , Man Feng 2 , Lin Su 1 , Wen Zhang 1, 3 , Huimei Chi 1, 2 , Degang Fu 1
RSC Advances ( IF 3.9 ) Pub Date : 2019-12-11 , DOI: 10.1039/c9ra08045g Tian Tian 1 , Xiaoyang Fan 1 , Man Feng 2 , Lin Su 1 , Wen Zhang 1, 3 , Huimei Chi 1, 2 , Degang Fu 1
Affiliation
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Flavin-mediated extracellular electron transfer was studied in two Gram-positive bacteria: Bacillus cereus strain DIF1 and Rhodococcus ruber strain DIF2. The electrochemical activities of these strains were confirmed using amperometric I–t curves and cyclic voltammetry (CV). Spent anodes with biofilms in fresh anolytes showed no redox peaks, while new anodes in the spent broth showed relative redox peaks using CV measurements, indicating the presence of a redox electron mediator secreted by bacteria. Adding riboflavins (RF) and flavin mononucleotide (FMN) improved the electron transfer of the microbial fuel cells inoculated with the two strains. The redox peaks indicated that flavins existed in the anolyte, and HPLC analysis showed that RF and FMN were secreted by the two bacterial strains. The concentration of RF increased until the bacteria grew to the log phase in microbial fuel cells. The concentration of RF decreased and that of FMN increased after the log phase. The two strains secreted FMN only in the microbial fuel cell. These results confirmed that the electrochemical activity mediated by flavins and FMN is essential in the extracellular electron transfer process in the strains DIF1 and DIF2.
中文翻译：
革兰氏阳性菌蜡状芽孢杆菌 DIF1 和红球菌 DIF2 中黄素介导的细胞外电子转移
在两种革兰氏阳性细菌中研究了黄素介导的细胞外电子转移：蜡状芽孢杆菌菌株 DIF1 和红球菌菌株 DIF2。这些菌株的电化学活性使用电流I - t确认曲线和循环伏安法（CV）。新鲜阳极液中具有生物膜的废阳极没有显示氧化还原峰，而使用 CV 测量的废液中的新阳极显示出相对的氧化还原峰，表明存在细菌分泌的氧化还原电子介质。添加核黄素 (RF) 和黄素单核苷酸 (FMN) 改善了接种这两种菌株的微生物燃料电池的电子转移。氧化还原峰表明阳极液中存在黄素类物质，HPLC分析表明两种菌株均分泌RF和FMN。RF 的浓度增加，直到细菌在微生物燃料电池中生长到对数期。对数期后RF浓度降低，FMN浓度升高。这两种菌株仅在微生物燃料电池中分泌 FMN。
更新日期：2019-12-11
中文翻译：
革兰氏阳性菌蜡状芽孢杆菌 DIF1 和红球菌 DIF2 中黄素介导的细胞外电子转移
在两种革兰氏阳性细菌中研究了黄素介导的细胞外电子转移：蜡状芽孢杆菌菌株 DIF1 和红球菌菌株 DIF2。这些菌株的电化学活性使用电流I - t确认曲线和循环伏安法（CV）。新鲜阳极液中具有生物膜的废阳极没有显示氧化还原峰，而使用 CV 测量的废液中的新阳极显示出相对的氧化还原峰，表明存在细菌分泌的氧化还原电子介质。添加核黄素 (RF) 和黄素单核苷酸 (FMN) 改善了接种这两种菌株的微生物燃料电池的电子转移。氧化还原峰表明阳极液中存在黄素类物质，HPLC分析表明两种菌株均分泌RF和FMN。RF 的浓度增加，直到细菌在微生物燃料电池中生长到对数期。对数期后RF浓度降低，FMN浓度升高。这两种菌株仅在微生物燃料电池中分泌 FMN。
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