二倍体和三倍体太平洋鲍鱼（Haliotis discus hannai; Gastropoda; Mollusca）男性的睾丸特异性丝氨酸/苏氨酸激酶1样（TSSK1样）基因的表征和表达模式。,PLOS ONE

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二倍体和三倍体太平洋鲍鱼（Haliotis discus hannai; Gastropoda; Mollusca）男性的睾丸特异性丝氨酸/苏氨酸激酶1样（TSSK1样）基因的表征和表达模式。
PLOS ONE ( IF 2.9 ) Pub Date : 2019-12-11 , DOI: 10.1371/journal.pone.0226022
Eun Jeong Kim ₁ , So Jeong Kim ₁ , Choul Ji Park ₂ , Yoon Kwon Nam ₁

Affiliation

睾丸特有的丝氨酸/苏氨酸激酶1样（TSSK1样）在后期精子发生和雄性育性中起重要作用，在太平洋鲍鱼Haliotis discus hannai（一种重要的商业海洋腹足动物）中得到了表征。此外，在二倍体和诱导三倍体雄性中评估了其表达模式，显示出睾丸成熟度的差异。类鲍鱼TSSK1与哺乳动物TSSK1s和其他潜在的后生直系同源基因共有保守的结构特征，尤其是在催化性STKc结构域方面。从系统发育上看，鲍鱼TSSK1样与软体动物TSSK1直系同源物和人类TSSK1表现出遗传联系。此外，鲍鱼TSSK1样基因显示出四部分外显子-内含子组织，这与大多数羊膜四足动物TSSK1的无内含子结构不同。后生动物谱系中的分子系统发育分析表明，最早祖传TSSK1的起源可能有变种。此外，鲍鱼TSSK1样显示睾丸占主导地位的表达，这受年龄和季节性繁殖周期的影响很大。在二倍体和三倍体鲍鱼雄性之间的比较表达分析表明，强健的TSSK1样表达主要发生在减数分裂后阶段。此外，RT-PCR分析表明，成熟的鲍鱼精子在释放后保留了TSSK1样转录物。两者合计，这项研究为进一步研究评估鲍鱼物种中雄性三倍体的雄性繁殖和不育和/或部分受精提供了有用的见识。鲍鱼TSSK1样表现出睾丸占优势的表达，受年龄和季节性繁殖周期的影响很大。在二倍体和三倍体鲍鱼雄性之间的比较表达分析表明，强劲的TSSK1样表达主要发生在减数分裂后阶段。此外，RT-PCR分析表明，成熟的鲍鱼精子在释放后保留了TSSK1样转录物。两者合计，这项研究为进一步研究评估鲍鱼物种中雄性三倍体的雄性繁殖和不育和/或部分受精提供了有用的见识。鲍鱼TSSK1样表现出睾丸占优势的表达，受年龄和季节性繁殖周期的影响很大。在二倍体和三倍体鲍鱼雄性之间的比较表达分析表明，强劲的TSSK1样表达主要发生在减数分裂后阶段。此外，RT-PCR分析表明，成熟的鲍鱼精子在释放后保留了TSSK1样转录物。两者合计，这项研究为进一步研究评估鲍鱼物种中雄性三倍体的雄性繁殖和不育和/或部分受精提供了有用的见识。在二倍体和三倍体鲍鱼雄性之间的比较表达分析表明，强劲的TSSK1样表达主要发生在减数分裂后阶段。此外，RT-PCR分析表明，成熟的鲍鱼精子在释放后保留了TSSK1样转录物。两者合计，这项研究为进一步研究评估鲍鱼物种中雄性三倍体的雄性繁殖和不育和/或部分受精提供了有用的见识。在二倍体和三倍体鲍鱼雄性之间的比较表达分析表明，强劲的TSSK1样表达主要发生在减数分裂后阶段。此外，RT-PCR分析表明，成熟的鲍鱼精子在释放后保留了TSSK1样转录物。两者合计，这项研究为进一步研究评估鲍鱼物种中雄性三倍体的雄性繁殖和不育和/或部分受精提供了有用的见识。

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Characterization of testis-specific serine/threonine kinase 1-like (TSSK1-like) gene and expression patterns in diploid and triploid Pacific abalone (Haliotis discus hannai; Gastropoda; Mollusca) males.

Testis-specific serine/threonine kinase 1-like (TSSK1-like), which plays important roles in late-phase spermatogenesis and male fertility, was characterized in Pacific abalone Haliotis discus hannai, an important commercial marine gastropod. Further, its expression patterns were assessed in diploid and induced triploid males showing differential degrees of testis maturation. Abalone TSSK1-like shared conserved structural features with mammalian TSSK1s and other potential metazoan orthologs, especially regarding the catalytic STKc domain. Phylogenetically, abalone TSSK1-like displayed a genetic affiliation with its molluscan TSSK1-like orthologs and human TSSK1. Additionally, abalone TSSK1-like gene showed a tetrapartite exon-intron organization, unlike the intronless structure of most amniotic tetrapodian TSSK1s. Molecular phylogenetic analysis in the metazoan lineage suggested a possible revision in the origin of the earliest ancestral TSSK1. Further, abalone TSSK1-like showed testis-predominant expression, which was significantly influenced by both age and seasonal reproductive cycles. Comparative expression analyses between diploid and triploid abalone males suggested that robust TSSK1-like expression occurred primarily at the post-meiotic stage. Additionally, RT-PCR assay indicates that mature abalone sperms retain TSSK1-like transcripts after release. Taken together, this study provides useful insights for further studies to assess male reproduction and sterility and/or partial fertility of induced male triploidy in abalone species.

更新日期：2019-12-11

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