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Tracing the mass flow from glucose and phenylalanine to pinoresinol and its glycosides in Phomopsis sp. XP-8 using stable isotope assisted TOF-MS.
Scientific Reports ( IF 3.8 ) Pub Date : 2019-12-06 , DOI: 10.1038/s41598-019-54836-1 Yan Zhang 1 , Junling Shi 2 , Yongqing Ni 1 , Yanlin Liu 3 , Zhixia Zhao 1 , Xixi Zhao 2 , Zhenhong Gao 2
Scientific Reports ( IF 3.8 ) Pub Date : 2019-12-06 , DOI: 10.1038/s41598-019-54836-1 Yan Zhang 1 , Junling Shi 2 , Yongqing Ni 1 , Yanlin Liu 3 , Zhixia Zhao 1 , Xixi Zhao 2 , Zhenhong Gao 2
Affiliation
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Phomopsis sp. XP-8, an endophytic fungus from the bark of Tu-Chung (Eucommia ulmoides Oliv) showed capability to biosynthesize pinoresinol (Pin) and pinoresinol diglucoside (PDG) from glucose (glu) and phenylalanine (Phe). To verify the mass flow in the biosynthesis pathway, [13C6]-labeled glu and [13C6]-labeled Phe were separately fed to the strain as sole substrates and [13C6]-labeled products were detected by ultra-high-performance liquid chromatography-quadrupole time of flight mass spectrometry. As results, [13C6]-labeled Phe was incorporated into [13C6]-cinnamylic acid (Ca) and p-coumaric acid (p-Co), and [13C12]-labeled Pin, which revealed that the Pin benzene ring came from Phe via the phenylpropane pathway. [13C6]-Labeled Ca and p-Co, [13C12]-labeled Pin, [13C18]-labeled pinoresinol monoglucoside (PMG), and [13C18]-labeled PDG products were found when [13C6]-labeled glu was used, demonstrating that the benzene ring and glucoside of PDG originated from glu. It was also determined that PMG was not the direct precursor of PDG in the biosynthetic pathway. The study identified the occurrence of phenylalanine- lignan biosynthesis pathway in fungi at the level of mass flow.
中文翻译：
追踪从拟南芥属中的葡萄糖和苯丙氨酸到松脂醇及其糖苷的质量流。XP-8使用稳定的同位素辅助TOF-MS。
拟南芥 XP-8是来自Tu-Chung（Eucommia ulmoides Oliv）树皮的内生真菌，具有从葡萄糖（glu）和苯丙氨酸（Phe）生物合成松脂醇（Pin）和松脂醇二葡萄糖苷（PDG）的能力。为了验证生物合成途径中的质量流量，将[13C6]标记的glu和[13C6]标记的Phe作为唯一底物分别喂入菌株，并通过超高效液相色谱法检测了[13C6]标记的产物，四极飞行时间质谱。结果，将[13C6]标记的Phe掺入[13C6]-肉桂酸（Ca）和对香豆酸（p-Co）和[13C12]标记的Pin中，这表明Pin苯环来自Phe通过苯丙烷途径。[13C6]标记的Ca和p-Co，[13C12]标记的Pin，[13C18]标记的松脂醇单糖苷（PMG），当使用[13C6]标记的glu时，发现了[13C18]标记的PDG产物，表明PDG的苯环和糖苷均源自glu。还确定了PMG不是生物合成途径中PDG的直接前体。该研究确定了真菌在质量流量水平上苯丙氨酸-木脂素生物合成途径的发生。
更新日期：2019-12-06
中文翻译：
追踪从拟南芥属中的葡萄糖和苯丙氨酸到松脂醇及其糖苷的质量流。XP-8使用稳定的同位素辅助TOF-MS。
拟南芥 XP-8是来自Tu-Chung（Eucommia ulmoides Oliv）树皮的内生真菌，具有从葡萄糖（glu）和苯丙氨酸（Phe）生物合成松脂醇（Pin）和松脂醇二葡萄糖苷（PDG）的能力。为了验证生物合成途径中的质量流量，将[13C6]标记的glu和[13C6]标记的Phe作为唯一底物分别喂入菌株，并通过超高效液相色谱法检测了[13C6]标记的产物，四极飞行时间质谱。结果，将[13C6]标记的Phe掺入[13C6]-肉桂酸（Ca）和对香豆酸（p-Co）和[13C12]标记的Pin中，这表明Pin苯环来自Phe通过苯丙烷途径。[13C6]标记的Ca和p-Co，[13C12]标记的Pin，[13C18]标记的松脂醇单糖苷（PMG），当使用[13C6]标记的glu时，发现了[13C18]标记的PDG产物，表明PDG的苯环和糖苷均源自glu。还确定了PMG不是生物合成途径中PDG的直接前体。该研究确定了真菌在质量流量水平上苯丙氨酸-木脂素生物合成途径的发生。
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