吡吡氧芬和代谢产物在大鼠肝脏中的毒性风险评估：一项体外研究。,Journal of Hazardous Materials

当前位置： X-MOL 学术 › J. Hazard. Mater. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

吡吡氧芬和代谢产物在大鼠肝脏中的毒性风险评估：一项体外研究。
Journal of Hazardous Materials ( IF 12.2 ) Pub Date : 2019-12-05 , DOI: 10.1016/j.jhazmat.2019.121835
Hui Liu ₁ , Peize Li ₁ , Peng Wang ₁ , Donghui Liu ₁ , Zhiqiang Zhou ₁

Affiliation

吡咯氧芬（PYR）是一种芳香族幼体激素类似物，是一种农业上用于控制昆虫物种的卫生杀虫剂。因此，评估PYR在哺乳动物中的代谢行为和毒性作用是评估其对人体健康的风险的最佳方法。先前的研究报道了关于PYR及其代谢物在大鼠肝细胞中的毒性风险的相互矛盾的结果。我们使用超高效液相色谱-串联质谱（UPLC-MS / MS）对PYR及其代谢物进行手性分析，以研究PYR在大鼠肝微粒体中的对映选择性代谢。我们的结果得出结论，PYR，代谢产物A和B的回收率范围为81.13％-111.54％，RSD值为0.01％-6.52％。PYR的方法检测限（LOD）和定量限（LOQ），代谢产物A和B符合分析要求。先前的研究表明PYR的对映选择性代谢和代谢产物的产生。对大鼠肝细胞的细胞增殖毒性，PYR及其代谢物诱导的凋亡和DNA损伤的测量表明，这些代谢物在大鼠肝细胞中具有比PYR更高的毒性潜能。在未来的工作中，迫切需要对PYR诱导的毒性的分子机制进行更多的研究。PYR及其代谢物在大鼠肝细胞中诱导的凋亡和DNA损伤表明，该代谢物在大鼠肝细胞中具有比PYR更高的毒性潜能。在未来的工作中，迫切需要对PYR诱导的毒性的分子机制进行更多的研究。PYR及其代谢物在大鼠肝细胞中诱导的凋亡和DNA损伤表明，该代谢物在大鼠肝细胞中具有比PYR更高的毒性潜能。在未来的工作中，迫切需要对PYR诱导的毒性的分子机制进行更多的研究。

"点击查看英文标题和摘要"

Toxicity risk assessment of pyriproxyfen and metabolites in the rat liver: A vitro study.

Pyriproxyfen (PYR) is a type of aromatic juvenile hormone analog and a hygienic insecticide used in agriculture to control insect species. Therefore, assessing the metabolic behavior and toxic effects of PYR in mammals is the best means of evaluating its risks to human health. Previous studies have reported conflicting results regarding the toxicity risks of PYR and its metabolites in rat hepatocytes. We used ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) to perform a chiral analysis of PYR and its metabolites investigating the enantioselective metabolism of PYR in rat liver microsomes. Our results concluded that the recoveries of PYR, metabolites A and B ranged from 81.13%-111.54 %, with RSD values of 0.01 %-6.52 %. The method limits of detection (LODs) and limits of quantification (LOQs) for PYR, metabolites A and B were in accordance with the analysis requirements. Previous studies have demonstrated the enantioselective metabolism of PYR and the generation of metabolites. Measurements of cell proliferation toxicity to rat hepatocytes, apoptosis and DNA damage induced by PYR and its metabolites in rat hepatocytes indicated that the metabolites reflected higher toxicity potential than PYR in rat hepatocytes. More studies about the molecular mechanism of PYR-induced toxicity are urgently needed in future work.

更新日期：2019-12-05

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南