Colorimetric determination of uric acid based on the suppression of oxidative etching of silver nanoparticles by chloroauric acid,Microchimica Acta

当前位置： X-MOL 学术 › Microchim. Acta › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Colorimetric determination of uric acid based on the suppression of oxidative etching of silver nanoparticles by chloroauric acid
Microchimica Acta ( IF 5.3 ) Pub Date : 2019-12-05 , DOI: 10.1007/s00604-019-4004-6
Li Li ₁ , Junli Wang ₁ , Zhengbo Chen ₂

Affiliation

A colorimetric method is described for the determination of uric acid (UA). The assay is based on oxidative etching of silver nanoparticles (AgNPs) by chloroauric acid (HAuCl4). The presence of UA suppresses the redox reaction between AgNPs and HAuCl4 because a competitive reaction occurs between HAuCl4 and UA. This results in a color change of the solution from brown to yellow. In parallel, the absorbance is blue shifted from 477 to 428 nm. The method has a detection limit as low as 30 pM (at S/N = 3) and a linear response range that covers the 0.1 nM to 0.1 mM UA concentration range. The reliability of the method was successfully demonstrated by analyzing spiked serum samples. Graphical abstract Schematic representation of a colorimetric method for determination of uric acid (UA) based on oxidative etching of silver nanoparticles by HAuCl4. Schematic representation of a colorimetric method for determination of uric acid (UA) based on oxidative etching of silver nanoparticles by HAuCl4.

中文翻译：

基于氯金酸抑制银纳米粒子氧化蚀刻的尿酸比色测定

描述了一种用于测定尿酸 (UA) 的比色法。该测定基于氯金酸 (HAuCl4) 对银纳米粒子 (AgNP) 的氧化蚀刻。UA 的存在抑制了 AgNPs 和 HAuCl4 之间的氧化还原反应，因为 HAuCl4 和 UA 之间发生了竞争反应。这导致溶液的颜色从棕色变为黄色。同时，吸光度从 477 nm 蓝移到 428 nm。该方法的检测限低至 30 pM（S/N = 3），线性响应范围涵盖 0.1 nM 至 0.1 mM UA 浓度范围。通过分析加标血清样品成功证明了该方法的可靠性。图形摘要基于 HAuCl4 氧化蚀刻银纳米颗粒的比色法测定尿酸 (UA) 的示意图。

更新日期：2019-12-05

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南