类胶原三螺旋结构的形成，稳定性和自组装行为：Ser，Ala和Arg / Glu的作用,ChemistrySelect

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类胶原三螺旋结构的形成，稳定性和自组装行为：Ser，Ala和Arg / Glu的作用
ChemistrySelect ( IF 1.9 ) Pub Date : 2019-12-05 , DOI: 10.1002/slct.201903500
Feiyi Shu ₁ , Chun Dai ₂ , Haibo Wang ₁ , Chengzhi Xu ₁ , Benmei Wie ₁ , Juntao Zhang ₁ , Yuling Xu ₁ , Lang He ₁ , Sheng Li ₁

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α链的独特序列是胶原三重螺旋的结构基石，决定了其构象和分子行为。在这项研究中，设计了具有不同序列特征的类胶原蛋白肽，以阐明Ser（极性残基），Ala（非极性残基）和Arg / Glu（可电离残基）在形成，动力学和稳定性方面的作用。三重螺旋及其自组装行为。我们发现（Gly-Hyp-Pro）_n中引入了其他常见氨基酸导致三重螺旋构象的形成速率和稳定性以及自组装成高阶结构的程度不同程度的降低。Ser的引入对这些过程表现出最大的有害作用，其次是Arg / Glu和Ala，带有Ser的胶原蛋白样肽无法形成热力学稳定的三螺旋构象，并丧失了进一步自我组装的能力。Arg / Glu促进了胶原样肽的自组装，而静电相互作用和氢键是类似于天然胶原的周期性有序结构自组装的关键。丙氨酸的疏水相互作用在某种程度上也促进了自组装，但是由于缺乏疏水相互作用的方向性，长程有序结构的形成受到了负面影响。

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Formation, Stability and Self‐Assembly Behaviour of the Collagen‐Like Triple Helix Confirmation: The Role of Ser, Ala and Arg/Glu

The unique sequence of the α‐chain is the structural cornerstone of the collagen triple helix that dictates its conformation and molecular behavior. In this study, collagen‐like peptides of varying sequence characteristics were designed to elucidate the role of Ser (polar residues), Ala (non‐polar residues), and Arg/Glu (ionizable residues) in the formation, kinetics, and stability of the triple helix, and its self‐assembly behavior. We found that the introduction of other common amino acids in (Gly‐Hyp‐Pro)_n results in varying degrees of decrease in the rate of formation and stability of the triple helix conformation and the self‐assembly into higher order structures. The introduction of Ser showed a maximal detrimental effect on these processes, followed by the Arg/Glu and Ala. Collagen‐like peptides with Ser could not form thermodynamically stable triple helix conformations and lost their ability to further self‐assemble. Arg/Glu promoted the self‐assembly of collagen‐like peptides, and electrostatic interactions and hydrogen bonding were key to the self‐assembly of periodic ordered structures similar to natural collagen. The hydrophobic interactions of Ala also accelerated self‐assembly to some extent, however, due to the lack of directionality of hydrophobic interactions, the formation of the long‐range ordered structure was affected negatively.

更新日期：2019-12-05

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