Transition-metal catalysis is a powerful tool for the construction of chemical bonds. Here we show that Pseudomonas savastanoi ethylene-forming enzyme (PsEFE), a non-heme iron enzyme, can catalyze olefin aziridination and nitrene C-H insertion, and that these activities can be improved by directed evolution. The non-heme iron center allows for facile modification of the primary coordination sphere by addition of metal-coordinating molecules, enabling control over enzyme activity and selectivity using small molecules.

中文翻译：

---

由非血红素铁酶催化并由非天然小分子配体增强的氮烯转移

过渡金属催化是构建化学键的有力工具。在这里，我们展示了 Pseudomonas savastanoi 乙烯形成酶 (PsEFE)，一种非血红素铁酶，可以催化烯烃氮丙啶化和氮烯 CH 插入，并且这些活性可以通过定向进化得到改善。非血红素铁中心允许通过添加金属配位分子轻松修饰初级配位球，从而能够使用小分子控制酶活性和选择性。