An impedimetric biosensor based on electrophoretically assembled ZnO nanorods and carboxylated graphene nanoflakes on an indium tin oxide electrode for detection of the DNA of Escherichia coli O157:H7,Microchimica Acta

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An impedimetric biosensor based on electrophoretically assembled ZnO nanorods and carboxylated graphene nanoflakes on an indium tin oxide electrode for detection of the DNA of Escherichia coli O157:H7
Microchimica Acta ( IF 5.3 ) Pub Date : 2019-12-03 , DOI: 10.1007/s00604-019-3921-8
Nandita Jaiswal ₁ , Chandra Mouli Pandey ₂ , Shipra Solanki ₃ , Ida Tiwari ₁ , Bansi Dhar Malhotra ₃

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Aminopropyltrimethoxysilane (APTMS)-functionalized zinc oxide (ZnO) nanorods and carboxylated graphene nanoflakes (c-GNF) were used in a composite that was electrophoretically deposited on an indium tin oxide (ITO) coated glass substrate. The modified ITO electrodes were characterized using various microscopic and spectroscopic techniques which confirm the deposition of the APTMS-ZnO/c-GNF composite. The electrodes have been used for the covalent immobilization of an Escherichia coli O157:H7 (E. coli)-specific DNA prob. Impedimetric studies revealed that the gene sensor displays linear response in a wide range of target DNA concentration (10−16 M to 10−6 M) with a detection limit of 0.1 fM. The studies on the cross-reactivity to other water-borne pathogens show that the bioelectrode is highly specific. Graphical abstract Schematic illustration for fabrication of nucleic acid biosensor for E. coli DNA detection using an ITO electrode modified with siloxane-functionalized zinc oxide (ZnO) nanorods and carboxylated graphene nanoflakes (c-GNFs). Schematic illustration for fabrication of nucleic acid biosensor for E. coli DNA detection using an ITO electrode modified with siloxane-functionalized zinc oxide (ZnO) nanorods and carboxylated graphene nanoflakes (c-GNFs).

中文翻译：

基于在氧化铟锡电极上电泳组装的 ZnO 纳米棒和羧化石墨烯纳米薄片的阻抗生物传感器，用于检测大肠杆菌 O157:H7 的 DNA

氨丙基三甲氧基硅烷 (APTMS) 功能化氧化锌 (ZnO) 纳米棒和羧化石墨烯纳米薄片 (c-GNF) 用于复合材料，该复合材料电泳沉积在氧化铟锡 (ITO) 涂层玻璃基板上。使用各种显微和光谱技术对修饰的 ITO 电极进行表征，这些技术证实了 APTMS-ZnO/c-GNF 复合材料的沉积。电极已用于共价固定大肠杆菌 O157:H7（大肠杆菌）特异性 DNA 探针。阻抗研究表明，基因传感器在广泛的目标 DNA 浓度范围内（10-16 M 至 10-6 M）显示线性响应，检测限为 0.1 fM。对其他水传播病原体的交叉反应性研究表明，该生物电极具有高度特异性。图形摘要使用用硅氧烷官能化氧化锌 (ZnO) 纳米棒和羧化石墨烯纳米薄片 (c-GNF) 修饰的 ITO 电极制造用于大肠杆菌 DNA 检测的核酸生物传感器的示意图。使用经硅氧烷官能化氧化锌 (ZnO) 纳米棒和羧化石墨烯纳米薄片 (c-GNF) 修饰的 ITO 电极制造用于大肠杆菌 DNA 检测的核酸生物传感器的示意图。

更新日期：2019-12-03

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