Inhibitory characteristics of flavonol-3-O-glycosides from Polygonum aviculare L. (common knotgrass) against porcine pancreatic lipase.,Scientific Reports

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Inhibitory characteristics of flavonol-3-O-glycosides from Polygonum aviculare L. (common knotgrass) against porcine pancreatic lipase.
Scientific Reports ( IF 3.8 ) Pub Date : 2019-12-02 , DOI: 10.1038/s41598-019-54546-8
Jun-Young Park ₁ , Chung Sun Kim ₁ , Kyung-Min Park ₂ , Pahn-Shick Chang _{1,

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Affiliation

Pancreatic lipase (PL) is an enzyme that plays an essential role in the digestion of dietary lipids and is a suitable target for an anti-obesity dietary supplement. The objective of this study was to find a novel source of PL inhibitors from Korean medicinal plants and investigate the PL-inhibitory properties of the active constituents. From among 34 kinds of methanolic crude extracts, Polygonum aviculare L. showed the highest PL-inhibitory activity (63.97 ± 0.05% of inhibition). Solvent fractionation and liquid chromatography/mass spectrometry (LC/MS) analysis identified flavonol-3-O-glycosides, flavonol-3-O-(2″-galloyl)-glycosides, and flavonol aglycones as active constituents. Furthermore, the inhibitory characteristics of the major compounds were investigated in terms of enzyme kinetics and fluorescence quenching. The results suggested that the inhibitory activity of the major compounds is closely related to the tertiary structural change in PL, and that differences in inhibitory activity occurred due to slight discrepancies in their chemical structure.

中文翻译：

虎杖（普通虎杖）黄酮醇-3-O-糖苷对猪胰脂肪酶的抑制特性。

胰腺脂肪酶（PL）是一种在饮食脂质的消化中起重要作用的酶，并且是抗肥胖饮食补充剂的合适靶标。这项研究的目的是从韩国药用植物中找到PL抑制剂的新来源，并研究活性成分的PL抑制特性。在34种甲醇粗提物中，何首乌表现出最高的PL抑制活性（抑制作用为63.97±0.05％）。溶剂分馏和液相色谱/质谱（LC / MS）分析确定了黄酮醇-3-O-糖苷，黄酮醇-3-O-（2″ -galloyl）-糖苷和黄酮醇苷元为活性成分。此外，从酶动力学和荧光猝灭方面研究了主要化合物的抑制特性。

更新日期：2019-12-02

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