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DNA-scaffold copper nanoclusters integrated into a cerium(III)-triggered Fenton-like reaction for the fluorometric and colorimetric enzymatic determination of glucose
Microchimica Acta ( IF 5.3 ) Pub Date : 2019-12-01 , DOI: 10.1007/s00604-019-4008-2
Hui Li 1 , Yuexiang Lu 2 , Jiawei Pang 1 , Jingwei Sun 1 , Fengyi Yang 1 , Ziyi Wang 1 , Yueying Liu 1
Affiliation  

AbstractA fluorometric and colorimetric method are described for the determination of hydrogen peroxide and glucose by integrating copper nanoclusters (CuNCs) into a Fenton-like reaction. The mechanism mainly depends on the fast formation of long-strand DNA-templated CuNCs with strong red fluorescence (with excitation/emission maxima at 340/640 nm) in the absence of H2O2. The DNA can be cleaved into short-oligonucleotide fragments by hydroxy radicals as formed in the Ce(III)-triggered Fenton-like reaction in the presence of H2O2. As a result, short-strand DNA loses the ability as a template for the formation of CuNCs. This leads to a decrease of fluorescence. The colorimetric assay, in turn, is based on the oxidation of colorless Ce(III) ions to the distinctly yellow Ce(IV) ions (with an absorption maximum at 400 nm) by H2O2. Compared with those assays based on the use of enzyme mimics, this method does not require any chromogenic substrates such as ABTS and TMB. Based on the dual-signal readout platform, we successfully achieved the detection of H2O2 and glucose. LODs are as low as 0.266 μM and 2.92 μM. The methods were applied to the sensitive determination of glucose by using glucose oxidase (GOx) which catalyzes the oxidization of glucose to produce H2O2. The practical application was demonstrated by determination of glucose in human serum, with apparent recoveries of 98.4–101.9% and 99.1–105.6%, respectively. The concentration of glucose ranges from 1 to 500 μM and 50 to 600 μM based on the dual-signal readout platform, respectively. This fluorometric and colorimetric dual-mode strategy will pave a new avenue for constructing effective assays for H2O2-related analytes in biochemical and clinical applications. Graphical abstractSchematic representation of a fluorometric and colorimetric dual-readout strategy for the sensitive determination of hydrogen peroxide and glucose. The assay has been designed by integrating copper nanoclusters into a Ce(III)-triggered Fenton-like reaction.

中文翻译:

将 DNA 支架铜纳米团簇集成到铈 (III) 触发的类芬顿反应中,用于葡萄糖的荧光和比色酶法测定

摘要描述了一种通过将铜纳米簇 (CuNCs) 整合到类芬顿反应中来测定过氧化氢和葡萄糖的荧光和比色法。该机制主要取决于在没有 H2O2 的情况下快速形成具有强红色荧光(激发/发射最大值为 340/640 nm)的长链 DNA 模板化 CuNCs。在 H2O2 存在下,在 Ce(III) 触发的类芬顿反应中形成的羟基自由基可以将 DNA 切割成短寡核苷酸片段。结果,短链 DNA 失去了作为形成 CuNCs 模板的能力。这导致荧光降低。反过来,比色测定基于 H2O2 将无色 Ce(III) 离子氧化为明显黄色的 Ce(IV) 离子(在 400 nm 处具有最大吸收)。与基于使用酶模拟物的那些测定相比,该方法不需要任何显色底物,如 ABTS 和 TMB。基于双信号读出平台,我们成功实现了H2O2和葡萄糖的检测。LOD 低至 0.266 μM 和 2.92 μM。该方法通过使用催化葡萄糖氧化生成 H2O2 的葡萄糖氧化酶 (GOx) 来灵敏测定葡萄糖。通过测定人血清中的葡萄糖证明了实际应用,表观回收率分别为 98.4-101.9% 和 99.1-105.6%。基于双信号读出平台,葡萄糖的浓度范围分别为 1 至 500 μM 和 50 至 600 μM。这种荧光和比色双模式策略将为构建有效的 H2O2 相关分析物在生化和临床应用中的分析铺平道路。图形摘要用于灵敏测定过氧化氢和葡萄糖的荧光和比色双读出策略的示意图。该测定是通过将​​铜纳米团簇整合到 Ce(III) 触发的类芬顿反应中来设计的。
更新日期:2019-12-01
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