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Mechanism of Pentagalloyl Glucose in Alleviating Fat Accumulation in Caenorhabditis elegans.
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2019-12-13 , DOI: 10.1021/acs.jafc.9b06167 Xiaoying Zhang 1 , Wei Li 1 , Yunzhou Tang 1 , Chunxiu Lin 1 , Yong Cao 1 , Yunjiao Chen 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2019-12-13 , DOI: 10.1021/acs.jafc.9b06167 Xiaoying Zhang 1 , Wei Li 1 , Yunzhou Tang 1 , Chunxiu Lin 1 , Yong Cao 1 , Yunjiao Chen 1
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Pentagalloyl glucose (PGG) has been studied for its valuable biological activities. However, the functional role of PGG in lipid metabolism in vivo is unclear. Here, we investigated the effects of PGG on lipid metabolism and its underlying mechanism in Caenorhabditis elegans. PGG decreased the accumulation of reactive oxygen species at 800 μM and remarkably increased the activities of antioxidant enzymes. PGG decreased significantly fat accumulation in wild-type worms (39.7 ± 5.7% in the normal group and 19.9 ± 4.5% in the high-fat group by Oil red O; 21.2 ± 2.7% in the high-fat group by Nile red; p < 0.001), but fat reduction by PGG was eliminated in the skn-1 mutant. The amount and size of lipid droplets in the ZXW618 mutant were decreased by PGG. The proportions of unsaturated fatty acids in both conditions were increased by PGG. In addition, the expression levels of fat metabolism genes were significantly changed in both conditions by PGG, which include mdt-15, pod-2, elo-2, fat-6, and fat-7 genes modulated fat synthesis; aak-2 and nhr-49 genes participated in fat consumption; and tub-1 gene regulated fat storage. However, fat-5 and acs-2 were downregulated in high-fat worms only, and vit-2 and lipl-4 were downregulated in normal worms only. Our study provided new insights into the role of PGG in alleviating fat accumulation and its underlying mechanism of action in C. elegans.
中文翻译：
戊铝酰葡萄糖减轻秀丽隐杆线虫脂肪积累的机制。
已经研究了戊五烷基葡萄糖（PGG）的宝贵生物活性。但是，PGG在体内脂质代谢中的功能作用尚不清楚。在这里，我们调查了PGG对秀丽隐杆线虫脂质代谢及其潜在机制的影响。PGG降低了800μM时活性氧的积累，并显着提高了抗氧化酶的活性。PGG显着降低了野生型蠕虫的脂肪积累（正常组为39.7±5.7％，高脂组为油红O，尼罗红为21.2±2.7％；高脂组为尼罗红21.2±2.7％； p <0.001），但在skn-1突变体中消除了PGG减少的脂肪。PGG降低了ZXW618突变体中脂质滴的数量和大小。在两种条件下，PGG均增加了不饱和脂肪酸的比例。此外，PGG在两种条件下均显着改变了脂肪代谢基因的表达水平，其中包括mdt-15，pod-2，elo-2，fat-6和fat-7基因调节脂肪合成。aak-2和nhr-49基因参与了脂肪的消耗。和tub-1基因调节脂肪的储存。但是，仅在高脂蠕虫中下调了fat-5和acs-2，仅在正常蠕虫中下调了vit-2和lipl-4。我们的研究提供了新的见解，PGG在减轻脂肪积累中的作用及其在秀丽隐杆线虫中的潜在作用机理。fat-5和acs-2仅在高脂蠕虫中被下调，而vit-2和lipl-4仅在正常蠕虫中被下调。我们的研究提供了新的见解，PGG在减轻脂肪积累中的作用及其在秀丽隐杆线虫中的潜在作用机理。fat-5和acs-2仅在高脂蠕虫中被下调，而vit-2和lipl-4仅在正常蠕虫中被下调。我们的研究提供了新的见解，PGG在减轻脂肪积累中的作用及其在秀丽隐杆线虫中的潜在作用机理。
更新日期：2019-12-17
中文翻译：
戊铝酰葡萄糖减轻秀丽隐杆线虫脂肪积累的机制。
已经研究了戊五烷基葡萄糖（PGG）的宝贵生物活性。但是，PGG在体内脂质代谢中的功能作用尚不清楚。在这里，我们调查了PGG对秀丽隐杆线虫脂质代谢及其潜在机制的影响。PGG降低了800μM时活性氧的积累，并显着提高了抗氧化酶的活性。PGG显着降低了野生型蠕虫的脂肪积累（正常组为39.7±5.7％，高脂组为油红O，尼罗红为21.2±2.7％；高脂组为尼罗红21.2±2.7％； p <0.001），但在skn-1突变体中消除了PGG减少的脂肪。PGG降低了ZXW618突变体中脂质滴的数量和大小。在两种条件下，PGG均增加了不饱和脂肪酸的比例。此外，PGG在两种条件下均显着改变了脂肪代谢基因的表达水平，其中包括mdt-15，pod-2，elo-2，fat-6和fat-7基因调节脂肪合成。aak-2和nhr-49基因参与了脂肪的消耗。和tub-1基因调节脂肪的储存。但是，仅在高脂蠕虫中下调了fat-5和acs-2，仅在正常蠕虫中下调了vit-2和lipl-4。我们的研究提供了新的见解，PGG在减轻脂肪积累中的作用及其在秀丽隐杆线虫中的潜在作用机理。fat-5和acs-2仅在高脂蠕虫中被下调，而vit-2和lipl-4仅在正常蠕虫中被下调。我们的研究提供了新的见解，PGG在减轻脂肪积累中的作用及其在秀丽隐杆线虫中的潜在作用机理。fat-5和acs-2仅在高脂蠕虫中被下调，而vit-2和lipl-4仅在正常蠕虫中被下调。我们的研究提供了新的见解，PGG在减轻脂肪积累中的作用及其在秀丽隐杆线虫中的潜在作用机理。
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