Magnetite nanoparticles coated with chitosan and polyethylenimine as anion exchanger for sorptive enrichment of phosphopeptides,Microchimica Acta

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Magnetite nanoparticles coated with chitosan and polyethylenimine as anion exchanger for sorptive enrichment of phosphopeptides
Microchimica Acta ( IF 5.3 ) Pub Date : 2019-11-28 , DOI: 10.1007/s00604-019-3971-y
Dilshad Hussain _{1,

2} , Syed Ghulam Musharraf ₂ , Batool Fatima ₃ , Adeela Saeed ₄ , Fahmida Jabeen ₄ , Muhammad Naeem Ashiq ₁ , Muhammad Najam-Ul-Haq _{1,

5}

Affiliation

AbstractAn anion exchange solid-phase sorbent is described. Chitosan coated magnetite nanoparticles were modified with polyethylenimine which is positively charged at pH 3 and therefore can be used for the magnet-supported enrichment of phosphopeptides which are negatively charged at this pH value. A 2-step strategy was used to synthesize the sorbent. The materials were characterized by transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetry and magnetic moment analysis. The anion exchanger was applied to extract phosphopeptides from a β-casein digest. Characteristic analytical figures include (a) a loading buffer of pH 3, (b) and elution buffer of pH 11, (c) a loading time of 5 min, (d) good selectivity (the β-casein to BSA ratio is 1:1000), and (e) excellent sensitivity (1 fmol). The optimized method was applied to egg yolk digest, non-fat milk digest, and diluted human serum. Graphical abstractSchematic representation of synthesis of PEI@chitosan@Fe3O4 nanoparticles, and of the enrichment of phosphopeptides by magnetic solid phase extraction prior to the determination of the peptides by MALDI-MS analysis.

中文翻译：

用壳聚糖和聚乙烯亚胺包覆的磁铁矿纳米粒子作为阴离子交换剂用于磷肽的吸附富集

摘要描述了一种阴离子交换固相吸附剂。壳聚糖包覆的磁铁矿纳米粒子被聚乙烯亚胺修饰，在 pH 值为 3 时带正电荷，因此可用于在此 pH 值下带负电荷的磷肽的磁体支持富集。使用两步策略来合成吸附剂。通过透射电子显微镜、扫描电子显微镜、傅里叶变换红外光谱、X射线衍射、热重分析和磁矩分析对材料进行了表征。阴离子交换剂用于从 β-酪蛋白消化物中提取磷酸肽。特征分析数据包括 (a) pH 3 的上样缓冲液，(b) 和 pH 11 的洗脱缓冲液，(c) 5 分钟的上样时间，(d) 良好的选择性（β-酪蛋白与 BSA 的比率为 1： 1000), (e) 出色的灵敏度 (1 fmol)。将优化后的方法应用于蛋黄消化物、脱脂牛奶消化物和稀释的人血清。图形摘要 PEI@壳聚糖@Fe3O4 纳米颗粒的合成示意图，以及在通过 MALDI-MS 分析测定肽之前通过磁性固相萃取富集磷酸肽的示意图。

更新日期：2019-11-28

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