化学治疗的4T1乳腺癌细胞的辐射响应通过使用红外激光阱的单细胞和多细胞电离来测量。,Scientific Reports

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化学治疗的4T1乳腺癌细胞的辐射响应通过使用红外激光阱的单细胞和多细胞电离来测量。
Scientific Reports ( IF 3.8 ) Pub Date : 2019-11-26 , DOI: 10.1038/s41598-019-53821-y
Endris Muhammed ₁ , Li Chen ₂ , Ying Gao ₃ , Daniel Erenso ₄

Affiliation

我们提出了一项研究，该研究使用激光捕获技术来测量未经处理和经化学处理的癌细胞的放射敏感性。我们使用了经过抗肿瘤化合物2-十二烷基-6-甲氧基环己-2、5-二烯-1,4-二酮（DMDD）处理的人乳腺肿瘤细胞系（4T1），该化合物是从杨桃（Averrhoa carambola L）的根中提取的在该研究中使用未处理的对照组以及2小时和24小时处理的4T1细胞组。吸收的阈值电离能（TIE）和阈值辐射剂量（TRD）是使用高功率红外激光（在1064 nm处）捕获，通过单细胞和多细胞捕获和电离来确定的。使用描述性和t统计量对结果进行了分析。与对照组相比，还分析了不同治疗时间下TIE和TRD与单个细胞质量的关系。随着治疗时间的增加，TIE和TRD均降低。但是，无论处理如何，TRD随质量而降低。每组中单细胞与多细胞电离的TRD分析也始终显示出相同的行为，而与处理无关。这些观察到的关系的潜在因素是根据辐射，热疗和化学效应来解释的。每组中单细胞与多细胞电离的TRD分析也始终显示出相同的行为，而与处理无关。这些观察到的关系的潜在因素是根据辐射，热疗和化学效应来解释的。每组中单细胞与多细胞电离的TRD分析也始终显示出相同的行为，而与处理无关。这些观察到的关系的潜在因素是根据辐射，热疗和化学效应来解释的。

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Chemo-treated 4T1 breast cancer cells radiation response measured by single and multiple cell ionization using infrared laser trap.

We present a study that uses a laser trapping technique for measurement of radiation sensitivity of untreated and chemo-treated cancer cells. We used a human mammary tumor cell line (4T1) treated by an antitumor compound, 2-Dodecyl-6-methoxycyclohexa-2, 5-diene-1,4-dione (DMDD), which was extracted from the root of Averrhoa carambola L. The untreated control group, and both 2-hour and 24-hour treated groups of 4T1 cells were used in this study. The absorbed threshold ionization energy (TIE) and the threshold radiation dose (TRD) were determined using a high-power infrared laser (at 1064 nm) trap by single and multiple cells trapping and ionization. The results were analyzed using descriptive and t-statistics. The relation of the TIE and TRD to the mass of the individual cells were also analyzed for different hours of treatment in comparison with the control group. Both TIE and TRD decrease with increasing treatment periods. However, the TRD decreases with mass regardless of the treatment. Analyses of the TRD for single vs multiple cells ionizations within each group have also consistently showed this same behavior regardless of the treatment. The underlying factors for these observed relations are explained in terms of radiation, hyperthermia, and chemo effects.

更新日期：2019-11-27

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