Characterization of a Histo-Blood Group Antigen-like Substance in Romaine Lettuce That Contributes to Human Norovirus Attachment.,Journal of Agricultural and Food Chemistry

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Characterization of a Histo-Blood Group Antigen-like Substance in Romaine Lettuce That Contributes to Human Norovirus Attachment.
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2019-12-06 , DOI: 10.1021/acs.jafc.9b05887
Zilei Zhang _{1,

2} , Danlei Liu _{1,

2} , Qingping Wu _{1,

2} , Yu Lu ₃ , Peng Tian ₄ , Zhongfu Wang ₃ , Dapeng Wang _{1,

2}

Affiliation

Human noroviruses (HuNoVs) are among the main pathogens causing acute nonbacterial gastroenteritis. Histo-blood group antigens (HBGAs) are widely accepted receptors for HuNoV specific binding. HBGA-like substances in produce are also considered as the critical ligands for capture of HuNoVs. However, the composition of viral ligands from food substrates remains unknown. In this study, an oligosaccharide (H2N2F2) was captured and isolated from romaine lettuce extract by a bacterial surface display system. Using electrospray ionization mass spectrometry and tandem mass spectrometry, it was shown that H2N2F2 was most likely to be a chimera of type A, H, and Lewis a HBGAs. The composition was consistent with our ELISA results using a panel of monoclonal antibodies against HBGAs. Our results revealed a possible interaction mechanism between HuNoVs and romaine lettuce. Better understanding of the interaction of HuNoVs with easily contaminated produce will ultimately aid in the control of and reduction in disease outbreaks.

中文翻译：

生菜中有助于人类诺如病毒附着的组织血型抗原样物质的表征。

人类诺如病毒（HuNoV）是引起急性非细菌性胃肠炎的主要病原体之一。组织血型抗原 (HBGA) 是广泛接受的 HuNoV 特异性结合受体。农产品中的 HBGA 类物质也被认为是捕获 HuNoV 的关键配体。然而，来自食品基质的病毒配体的组成仍然未知。在本研究中，通过细菌表面展示系统从生菜提取物中捕获并分离出寡糖 (H2N2F2)。利用电喷雾电离质谱和串联质谱分析表明，H2N2F2最有可能是A型、H型和Lewis a HBGAs的嵌合体。该组合物与我们使用一组抗 HBGA 单克隆抗体的 ELISA 结果一致。我们的结果揭示了 HuNoV 和生菜之间可能的相互作用机制。更好地了解 HuNoV 与易污染农产品的相互作用最终将有助于控制和减少疾病爆发。

更新日期：2019-12-07

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