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The interaction of CaM7 and CNGC14 regulates root hair growth in Arabidopsis.
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-01-29 , DOI: 10.1111/jipb.12890 Qudsia Zeb 1 , Xiaohan Wang 1 , Congcong Hou 1 , Xiwen Zhang 1 , Mengqi Dong 1 , Sisi Zhang 1 , Qian Zhang 1 , Zhijie Ren 1 , Wang Tian 1 , Huifen Zhu 1 , Legong Li 1 , Liangyu Liu 1
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-01-29 , DOI: 10.1111/jipb.12890 Qudsia Zeb 1 , Xiaohan Wang 1 , Congcong Hou 1 , Xiwen Zhang 1 , Mengqi Dong 1 , Sisi Zhang 1 , Qian Zhang 1 , Zhijie Ren 1 , Wang Tian 1 , Huifen Zhu 1 , Legong Li 1 , Liangyu Liu 1
Affiliation
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Oscillations in cytosolic free calcium determine the polarity of tip‐growing root hairs. The Ca2+ channel cyclic nucleotide gated channel 14 (CNGC14) contributes to the dynamic changes in Ca2+ concentration gradient at the root hair tip. However, the mechanisms that regulate CNGC14 are unknown. In this study, we detected a direct interaction between calmodulin 7 (CaM7) and CNGC14 through yeast two‐hybrid and bimolecular fluorescence complementation assays. We demonstrated that the third EF‐hand domain of CaM7 specifically interacts with the cytosolic C‐terminal domain of CNGC14. A two‐electrode voltage clamp assay showed that CaM7 completely inhibits CNGC14‐mediated Ca2+ influx, suggesting that CaM7 negatively regulates CNGC14‐mediated calcium signaling. Furthermore, CaM7 overexpressing lines phenocopy the short root hair phenotype of a cngc14 mutant and this phenotype is insensitive to changes in external Ca2+ concentrations. We, thus, identified CaM7‐CNGC14 as a novel interacting module that regulates polar growth in root hairs by controlling the tip‐focused Ca2+ signal.
中文翻译：
CaM7和CNGC14的相互作用调节拟南芥中根毛的生长。
胞质游离钙的振荡决定了尖端生长的根毛的极性。Ca 2+通道环状核苷酸门控通道14（CNGC14）有助于根发尖处Ca 2+浓度梯度的动态变化。但是，调节CNGC14的机制尚不清楚。在这项研究中，我们通过酵母双杂交和双分子荧光互补测定法检测到钙调蛋白7（CaM7）与CNGC14之间的直接相互作用。我们证明了CaM7的第三个EF手结构域与CNGC14的胞质C末端结构域特异性相互作用。两电极电压钳分析表明CaM7完全抑制CNGC14介导的Ca 2+大量涌入，表明CaM7负调节CNGC14介导的钙信号传导。此外，CaM7过表达系表型复制cngc14突变体的短根毛表型，并且该表型对外部Ca 2+浓度的变化不敏感。因此，我们将CaM7-CNGC14确定为一种新型的相互作用模块，该模块通过控制尖端聚焦的Ca 2+信号来调节根毛中的极性生长。
更新日期：2020-01-29
中文翻译：
CaM7和CNGC14的相互作用调节拟南芥中根毛的生长。
胞质游离钙的振荡决定了尖端生长的根毛的极性。Ca 2+通道环状核苷酸门控通道14（CNGC14）有助于根发尖处Ca 2+浓度梯度的动态变化。但是，调节CNGC14的机制尚不清楚。在这项研究中，我们通过酵母双杂交和双分子荧光互补测定法检测到钙调蛋白7（CaM7）与CNGC14之间的直接相互作用。我们证明了CaM7的第三个EF手结构域与CNGC14的胞质C末端结构域特异性相互作用。两电极电压钳分析表明CaM7完全抑制CNGC14介导的Ca 2+大量涌入，表明CaM7负调节CNGC14介导的钙信号传导。此外，CaM7过表达系表型复制cngc14突变体的短根毛表型，并且该表型对外部Ca 2+浓度的变化不敏感。因此，我们将CaM7-CNGC14确定为一种新型的相互作用模块，该模块通过控制尖端聚焦的Ca 2+信号来调节根毛中的极性生长。
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